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Bacillus sp. strain with antifungal, antibacterial and growth promotion activity

A technology of bacillus and strains, applied in the direction of plant growth regulators, microbial-based methods, bacteria, etc., can solve the problems of narrowing the range of pesticides and harmful environmental impact of pesticides, etc.

Active Publication Date: 2015-06-17
MARRONE BIO INNOVATIONS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The range of available insecticides narrows as resistance to increasing chemical insecticides develops
In addition, non-naturally occurring pesticides may have harmful environmental effects

Method used

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  • Bacillus sp. strain with antifungal, antibacterial and growth promotion activity
  • Bacillus sp. strain with antifungal, antibacterial and growth promotion activity
  • Bacillus sp. strain with antifungal, antibacterial and growth promotion activity

Examples

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Effect test

example 1

[0149] Example 1: Isolation and characterization of Bacillus isolate F727 by 16S rRNA, recA and phoR sequences

[0150] Bacillus strain F727 was isolated from soil samples collected in Jonesville, California (CA) using the traditional plate dilution method. Isolates were identified as Flavobacterium by PCR amplification and sequencing of the 16S rRNA, recA and phoR genes using Universal Bacterial primers. (2008) International System Bacteriology and Purification (Int.J.Sys.Evol Microbiol.) 58:919-923; Guo (Guo) et al. (2012) Canadian Journal of Microbiology (Can J. Microbiol) 58:1295-1305.

[0151] Growth was scraped from the 24 hour potato dextrose plates with a sterile loop and resuspended in DNA extraction buffer. DNA was extracted using MoBio Ultra Clean Microbial DNA Extraction Kit. Check the quality / quantity of the DNA extract by electrophoresis of a 5uL aliquot on a 1% agarose gel.

[0152] rRNA sequence

[0153]By mixing 2 μL of clean DNA extract with 25 μL of G...

example 2

[0188] Example 2: Fatty acid composition of isolate F727

[0189] Fatty acid profiling of isolate F727 was performed at MIDI Labs, Inc (Newark, DE) according to commercial standards. The results are shown in Table 2. Comparison of its fatty acid expression profile with the RTSBA6 6.10 fatty acid database revealed that isolate F727 had a similarity index of 0.885 to Bacillus subtilis.

[0190] Table 2

[0191]

example 3

[0192] Example 3: Characterization of isolate F727 by MALDI-TOF protein expression profiling

[0193] MALDI-TOF mass spectrometry protein profiling of isolate F727 was performed at MIDI Laboratories Inc. (Newark, DE). Isolate F727 displayed a MALDI-TOF protein expression profile distinct from that of any other microorganism present in the mass spectrometry database. Some similarities were observed to the protein expression profiles of B. death valley, B. moeri and B. subtilis; however, none of the similarity scores were high enough to indicate even a universal match.

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Abstract

Disclosed herein is a Bacillus strain, Bacillus sp. isolate F727, that produces metabolites with pesticidal activities. Also provided are bioactive compositions and metabolites derived from cultures of Bacillus sp. isolate F727 capable of controlling pests; as well as methods of use of the strain and its metabolites for controlling pests.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application No. 61 / 683,174, filed August 14, 2012, and U.S. Patent Application No. 13 / 835,677, filed March 15, 2013; disclosure of said patent applications The content is hereby incorporated by reference in its entirety for all purposes. technical field [0003] The present invention is in the field of biopesticides and pest control; in particular microbial pesticides and microbial strains producing said microbial pesticides. Background technique [0004] Natural products are substances produced by microorganisms, plants and other organisms. Microbial natural products provide a rich source of chemical diversity, and there is a long history of utilizing natural products for medicinal purposes. Despite the emphasis on natural products for human therapeutics, over 50% of which are derived from natural products, only 11% of pesticides are derived from natura...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/22
CPCA01N63/00C12N1/20A01N63/22C12R2001/07C12N1/205A01N65/30
Inventor 拉特纳卡·阿索尔卡安娜·露西娅·科尔多瓦-科雷洛斯克里斯托弗·麦科特德博拉·维尔克卡尔利·托德苏海帕梅拉·马罗内
Owner MARRONE BIO INNOVATIONS