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Method for improving transfer embryo use ratio of multiparous mammal

A mammalian and embryo transfer technology, applied in the field of mammalian embryo transfer, can solve the problems such as insufficient utilization of cloned embryos, and achieve the effects of reducing the number, reducing the cost and reducing the implantation.

Inactive Publication Date: 2015-07-01
BGI ARK BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To obtain cloned animals with multiple fetuses, a certain number of embryos is required to allow the surrogate recipient to conceive and give birth. Simple transplantation of cloned embryos causes many cloned embryos that can develop into individuals to degenerate due to nutritional competition in the later stage, and cloned embryos cannot be fully utilized.

Method used

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  • Method for improving transfer embryo use ratio of multiparous mammal
  • Method for improving transfer embryo use ratio of multiparous mammal
  • Method for improving transfer embryo use ratio of multiparous mammal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] According to the data of our laboratory, the number of cloned pig embryos transferred in the range of 95±5 has the best pregnancy rate and delivery rate, so a certain number of PA embryos are added in this range to conduct experiments to study the effect of PA embryos on cloned embryo transfer of multiple-fetal animals Whether the effect is better.

[0069]

[0070] The results showed that within the range of the same number of transplanted embryos, adding PA embryos relatively reduced the number of HMC embryos, did not reduce the transplantation effect, and even improved the transplantation efficiency. After adding PA embryos, the delivery rate and litter size of transplanted recipients were improved, and the utilization rate of cloned embryos (litter size / HMC transplanted number) increased to 2.5 times of the original.

Embodiment 2

[0072] Cloned embryos and parthenogenetic activated embryos were obtained according to the experimental steps in the technical scheme. The total number of transplanted embryos is within the range of 95±10, and the parthenogenetic activated embryos accounting for 10%, 20%, 30%, and >40% of the total embryos transferred are respectively transplanted to different surrogate recipients, and the delivery rate and delivery rate of the surrogate recipients are observed and compared. Average litter size. The results are shown in Table 2.

[0073] Table 2 Mixed transfer data of different proportions of parthenogenetic activated embryos

[0074]

[0075] Note: Different letters a and b in the same column indicate extremely significant difference, P<0.01

[0076] >40% of the data contain proportions from about 0% to about 50%

[0077] The data show that when parthenogenetically activated embryos account for about 30% of the number of transferred embryos, the delivery rate and averag...

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Abstract

The invention discloses a method for improving transfer embryo use ratio of a multiparous mammal. Parthenogenetic activation blastaea which cannot grow to individuals and cloned embryos are implanted together to maximize the use ratio of the cloned embryos.

Description

technical field [0001] The invention relates to the field of mammalian embryo transplantation, in particular to a method for improving the utilization rate of transplanted embryos in multiple-born mammals. Background technique [0002] Animal cloning is one of the important technical means commonly used to obtain transgenic animals. The cloning technology makes the genetic material of the egg cell ineffective by physically removing the nucleus of the egg cell or by chemical methods (Fulka and Moor, 1993), transfers a single nucleated cell into the egg cell by electric shock or direct microinjection, and then physically or chemically transfers the egg cell to the egg cell. The reconstructed embryos are activated to develop into embryos and transplanted into surrogate mothers to obtain cloned individuals. [0003] In 1996, British scientist Wilnut used adult sheep mammary gland cells as nuclear donors to clone sheep Dolly from somatic cells for the first time (Wilmut et al., ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/04
CPCA61D19/04
Inventor 张译捷窦红伟徐颖杨珍珍翟珊莉杜玉涛
Owner BGI ARK BIOTECH
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