A kind of Paecilomyces variotii strain sj1 and its application

A technology of Paecilomyces variotii and strains, applied in the fields of application, fungi, chemicals for biological control, etc., can solve the problems of accelerated species disappearance, frequent sand and dust, water pollution, etc., and achieve the promotion of rhizosphere The effect of microbial growth, promotion of root growth, and promotion of growth and development

Active Publication Date: 2017-07-11
SHANDONG PENGBO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unrestrained pressure on the agricultural ecosystem results in the transfer of pressure to the entire ecosystem, leading to water pollution, frequent occurrence of sand and dust, accelerated species disappearance, etc.

Method used

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  • A kind of Paecilomyces variotii strain sj1 and its application
  • A kind of Paecilomyces variotii strain sj1 and its application
  • A kind of Paecilomyces variotii strain sj1 and its application

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Effect test

Embodiment 1

[0021] Embodiment 1 Paecilomyces variotii (Paecilomyces variotii) Isolation and Identification of Strain SJ1

[0022] (1) Isolation of endophytic fungi from Seabuckthorn

[0023] ①The healthy wild seabuckthorn samples are processed and washed immediately after collection, then the roots are cut into small pieces, rinsed with 75% alcohol, washed with 10% pasteurized solution for 18 minutes, and finally rinsed with sterile water for 4 times;

[0024] ② Cut the above-mentioned sterilized roots into 0.5cm-long sections under aseptic conditions and inoculate them on the Bengal red medium plate, and place them in an incubator at 28°C for 5 days. Bacteria can be seen growing from the cut edges of the samples. Silk, separated and purified repeatedly on a plate (Red Bengal medium), and finally Paecilomyces variotii was obtained (Paecilomyces variotii) Bacterial strain SJ1; Red Bengal medium formula and preparation method are as follows: peptone 5g, glucose 10g, potassium dihydrogen p...

Embodiment 2

[0029] Embodiment 2 Paecilomyces variotii (Paecilomyces variotii) Growth-promoting experiment of strain SJ1

[0030] (1) Liquid fermentation culture of seabuckthorn endophytic fungi:

[0031] Paecilomyces variotii isolated from sea buckthorn endophytic fungi (Paecilomyces variotii) Strain SJ1 was placed on the plate PDA medium, cultured at 25°C for 6 days, and inoculated into a 250mL Erlenmeyer flask containing 50mL seed culture solution (made of PDA medium without agar) with a puncher agar, at 28°C , cultivated on a rotary shaker at 120r / min for 3 days as seeds, inoculated into 150mL fermentation medium (fermentation medium: potato extract 1.0 L, yeast extract 1.0 g, peptone 3.0 g, glucose 15.0 g , agar 17.0 g, preparation of potato extract: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of sea water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1000 ml.) into a 500 mL Erlenmeyer flask, Cultivate under the same co...

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PUM

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Abstract

The invention discloses a strain of Paecilomyces variotii (Paecilomyces variotii) SJ1, which is a seabuckthorn fungus, a preparation method of the seabuckthorn endophytic fungus and its extract, and an application of the seabuckthorn fungus extract to promote the growth of corn, rice and other crops, Root development, improve frost resistance, increase yield. The SJ1 strain was identified as Paecilomyces variotii according to its morphological and genetic analysis.

Description

technical field [0001] The invention relates to the field of biological technology, specifically a Paecilomyces variotii (Paecilomyces variotii) Strain SJ1 and its application. Background technique [0002] With the expansion of population and the improvement of people's living standards, people's demand for agricultural products is increasing. China is a country with a large population, with less than 2 mu of cultivated land per capita. The perennial predatory production and management and backward technology have overwhelmed the agricultural ecosystem. Soil degradation, soil erosion, excessive use of chemical pesticides and fertilizers, and frequent occurrence of extreme weather Seriously affect the safety of my country's grain production, as an important resource material, grain cannot depend on the international market. The unrestrained pressure on the agricultural ecosystem results in the transfer of the pressure to the entire ecosystem, leading to water pollution, fre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/79
CPCA01N63/30C12N1/145C12R2001/79
Inventor 储昭辉张民丁新华王英姿王庆彬王洪凤耿全政
Owner SHANDONG PENGBO BIOTECHNOLOGY CO LTD
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