Method for preparing feed protein powder and fermentation nutrition accelerator by comprehensive utilization of corn soaking water
A technology of corn soaking water and nutrient accelerators, applied in fermentation, protein food ingredients, protein food processing, etc., can solve the problems of low utilization and narrow application
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[0038] The present invention will be further described below in conjunction with the examples, but not limited thereto. The content % in the embodiment is all mass percent.
[0039] The raw material in the embodiment is the corn soaking water of the sulfurous acid countercurrent soaking process produced by corn starch, which is taken from Luzhou Biotechnology (Shandong) Co., Ltd.; the phytase in the embodiment is a liquid enzyme, purchased from Wuhan New Huayang Biological Co., Ltd. , Enzyme activity 5000U / mL. The ceramic membranes, 4040 ultrafiltration membranes, and 4040 nanofiltration membranes used in the examples are all commonly used products in this field and are available in the market.
[0040] Terminology Explanation: Ultrafiltration membrane cut-off pore diameter 5000D, 8000D refers to ultrafiltration membrane pore diameter cut-off molecular weight 5000 Dalton, 8000 Dalton, referred to as cut-off pore size 5000D, cut-off pore size 8000D.
[0041] The equipment use...
Embodiment 1
[0045] Embodiment 1, the method for comprehensive utilization of corn soaking water to produce feed protein powder and fermentation nutrition accelerator, the steps are as follows:
[0046] (1) Enzymatic hydrolysis of phytic acid in soaking water
[0047] Measure the volume L of corn soaking water, add phytase according to 1000U / L, and hydrolyze for 5 hours at a temperature of 45°C. The detected phytic acid content is 0.12%, and the hydrolysis rate of phytic acid is 90%.
[0048] (2) Microfiltration
[0049] The soaking water after enzymolysis is pretreated with a microfiltration membrane. The microfiltration membrane is a ceramic membrane (Anhui Pulang Membrane Separation Co., Ltd.), with a pore size of 0.2 microns, a filtration pressure of 2-4MPa, and an initial clear liquid flow rate of 2L / min. When the clear liquid flow rate drops to 0.4L / min, the separation is stopped, and the mother liquid (impermeable liquid) and clear liquid (permeate liquid) are collected respectivel...
Embodiment 2
[0069] Embodiment 2, the method for comprehensive utilization of corn soaking water to produce feed protein powder and fermentation nutrition accelerator, the steps are as follows:
[0070] (1) Enzymatic hydrolysis of phytic acid in soaking water
[0071] Accurately measure the volume of corn soaking water, add phytase at 1500U / L, and hydrolyze for 4 hours at a temperature of 40°C. The detected phytic acid content is 0.15%, and the hydrolysis rate of phytic acid is 87.5%.
[0072] (2) Microfiltration
[0073] The soaking water after enzymatic hydrolysis is pretreated by microfiltration membrane. The microfiltration membrane is a ceramic membrane with a pore size of 0.5 microns and a filtration pressure of 2-3MPa. The initial clear liquid flow rate is 2.4L / min. , stop the separation, respectively collect the mother liquor (non-permeable liquid) and clear liquid (permeate liquid), and the main components of the mother liquor are insoluble protein particles.
[0074] (3) ultraf...
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