Fermentation brewer grains for partially replacing bean pulp in broiler geese feed
A technology for meat goose feed and brewer's grains, applied in the field of fermented brewer's grains, can solve problems such as narrow application range, poor palatability, waste of environment, etc., and achieve the effects of increasing average body weight, increasing feed intake, and wide sources
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[0016] Example 1:
[0017] A preparation method of fermented brewer's grain used to partially replace soybean meal in meat goose feed includes the following steps:
[0018] (1) Test preparation:
[0019] Source of Bacillus subtilis: Culture Collection Center of Institute of Microbiology, Chinese Academy of Sciences, Bacillus subtilis subsp. subtilis, AS1.2163; Source of Yeast: Culture Collection Center of Institute of Microbiology, Chinese Academy of Sciences, Saccharomyces cerevisiae, AS2.3875.
[0020] Weigh 18.0 g of nutrient broth, heat it and dissolve it in 1000 mL of distilled water, with a pH of 7, dispense each 100 mL in a triangular flask, and autoclave at 121°C for 25 minutes for use.
[0021] Turn on the UV lamp of the ultra-clean workbench for 30 minutes, then turn on the fan to blow for 10 minutes; wait until the temperature of the bacterial solution returns to room temperature for inoculation; pick a loop of Bacillus subtilis in the ultra-clean workbench and inoculate it ...
Example Embodiment
[0029] Example 2:
[0030] A preparation method of fermented brewer's grain used to partially replace soybean meal in meat goose feed includes the following steps:
[0031] (1) Preparation of fermentation medium: Weigh 2.5 kg of brewer’s grains, 1.2 kg of corn flour, 0.8 kg of bran, and 2.4 kg of distilled water. Then add 0.016 kg of brown sugar, 0.024 kg of beef extract, and 0.008 kg of ammonium sulfate to supplement the nutritional deficiency , And mix them evenly;
[0032] (2) Inoculation: Add Bacillus subtilis broth (2.8×10 8 CFU / mL) and activated Saccharomyces cerevisiae (1.3×10 9 CFU / g), the inoculum amount is 16mL / 100g and 12g / 100g respectively, then mix well, put it in a sealed container, and incubate at 32°C for 10 days.
Example Embodiment
[0033] Example 3:
[0034] A preparation method of fermented brewer's grain used to partially replace soybean meal in meat goose feed includes the following steps:
[0035] (1) Preparation of fermentation medium: Weigh 3.5 kg of brewer’s grains, 0.8 kg of corn flour, 1.2 kg of bran, 1.6 kg of distilled water, and add 0.024 kg of brown sugar, 0.016 kg of beef extract, and 0.012 kg of ammonium sulfate to supplement nutritional deficiency , And mix them evenly;
[0036] (2) Inoculation: Add Bacillus subtilis broth (2.8×10 8 CFU / mL) and activated Saccharomyces cerevisiae (1.3×10 9 CFU / g), the inoculum amount is 16mL / 100g and 12g / 100g respectively, then mix evenly, put it in a sealed container, and incubate at 32°C for 12 days.
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