Subcritical water extraction agent and extraction method for polyphenols in lotus solid waste

A solid waste and subcritical water technology, applied in solid solvent extraction, organic chemistry, etc., can solve problems such as patents that have not been reported, and achieve the effect of simple extraction process, high extraction yield and low viscosity

Inactive Publication Date: 2016-08-17
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many research reports on the use of subcritical water extraction to pretreat environmental samples, sewage and soil for analysis. In recent years, subcritical water has been used to extract natural active ingredients such as volatile oil, flavonoids, polyphenols, and mushroomene. , saponins and anthrone substances, but there is no report on the subcritical water extraction of lotus solid waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The composition and ratio of the extractant in parts by weight are: citric acid 1; Vc 0.1; sodium bisulfite 0.05, domestic water to 100%.

[0027] Take the processed fresh lotus root skin of the lotus root, clean it, filter water, without crushing, directly take 500kg of material and add it to the extraction kettle immediately, pump 500kg of the above-mentioned extraction agent, fill it with nitrogen for 15min, seal it, control the pressure of the extraction kettle at 8MPa, After the extraction temperature was 180° C., the extraction time was 10 minutes, cooled to 30° C., centrifuged at 3000 r / min, and the supernatant was collected. Pump the supernatant into the AB-8 macroporous adsorption resin column at a flow rate of 20kg / min. After equilibrating for 30min, wash with distilled water to remove unadsorbed impurities, then elute the resin column with 70% ethanol aqueous solution, and collect The eluate was recovered from ethanol under reduced pressure at 40°C to obtain ...

Embodiment 2

[0029] The composition and ratio of the extractant in parts by weight are: malic acid 1; Vc 1; sodium bisulfite 0.04; domestic water supplemented to 100%.

[0030] Take the processed fresh lotus root joints, clean them, filter water, primary crush, directly take out 500kg of material and add it to the extraction kettle immediately, pump 1000kg of the above extraction agent, fill it with nitrogen for 15min, seal it, control the pressure of the extraction kettle at 10MPa, and put it into the extraction kettle. After extracting for 20 minutes at a temperature of 160°C, cool to 30°C, centrifuge at 3000r / min, and collect the supernatant. Pump the supernatant into the AB-8 macroporous adsorption resin column at a flow rate of 20kg / min. After equilibrating for 30min, wash with distilled water to remove unadsorbed impurities, then elute the resin column with 70% ethanol aqueous solution, and collect Eluate, ethanol is recovered under reduced pressure at 40°C to obtain a concentrated s...

Embodiment 3

[0032] The composition and ratio of the extractant in parts by weight are: citric acid 0.5, malic acid 0.5; Vc 0.4; sodium bisulfite 0.4, domestic water to 100%.

[0033]Take the freshly deseeded lotus pod, clean it, filter water, roughly cut it, take 500kg of material and add it to the extraction kettle immediately, pump 800kg of the above extraction agent, fill it with nitrogen for 15 minutes, seal it, control the pressure of the extraction kettle at 5MPa, and extract it at the extraction temperature After the extraction time is 10min at 180°C, cool to 30°C, enrich / separate and dry according to the method of Example 1 to obtain 12.5kg of lotus pod polyphenols with a polyphenol content of 90.1%, including 10kg of proanthocyanidins and a content of proanthocyanidins was 93.6%.

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PUM

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Abstract

The invention discloses a subcritical water extraction agent and a method for extracting polyphenol substances from lotus solid wastes. The subcritical water extraction agent is prepared from water, citric acid or malic acid or a mixture of the citric acid and the malic acid, Vc and sodium bisulfate according to certain weight parts. The extraction method comprises the steps of extracting for a fixed time by controlling pressure and temperature, centrifugally filtering to obtain filtrate, separating / enriching the filtrate by macroporous resin, eluting with ethanol, recovering a solvent and drying the concentrated liquid to obtain the polyphenol substances of the lotus solid wastes. The extraction agent disclosed by the invention can restrain the activity of polyphenol oxidase, can increase the leaching rate of the polyphenol substances during extraction by combining with subcritical water, and shortens the operation time; and the extraction agent and the method disclosed by the invention have no remaining of the solvent during extraction, are environmentally friendly and are easily industrialized.

Description

technical field [0001] The invention belongs to the technical field of processing agricultural products and their by-products, and relates to the resource utilization technology of solid waste, in particular to a subcritical water extraction agent and an extraction method for polyphenols in lotus solid waste. Background technique [0002] Lotus (Nelumbo Nucifera Gaertn) is a large perennial aquatic herbaceous plant of the family Nymphaeaceae. It is native to China and India. In my country, it is mainly distributed in the shallow water areas of the Yangtze River, Yellow River, Pearl River and freshwater lakes. Both lotus root and lotus seeds are rich in nutrients, and are well-known delicacies with high health value. Therefore, in recent years, the planting area of ​​lotus root in my country has reached more than 7 million mu, and the annual output of lotus root is nearly 100 million tons. However, the annual output of solid waste in the production and processing of lotus roo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02C07G99/00
Inventor 段玉清罗孝平张海晖程燕翔张迪蔡梅红何远清
Owner JIANGSU UNIV
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