Sterile micro grafting method of koelreuteria bipinnata var. intergrifoliola 'Jinyan'

A technology of golden flame colorful luan and micro-grafting, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as affecting subculture, difficulty in mastering operation techniques, and high survival rate, so as to improve survival rate and The effect of the germination effect

Inactive Publication Date: 2015-07-22
JIANGSU ACAD OF FORESTRY
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AI Technical Summary

Problems solved by technology

[0003] Jinyan Cailuan (Koelreuteria bipinnata var. integrifoliola 'Jinyan') is an excellent variety selected from Huangshan eucalyptus. Its main characteristics are that the trunk or branches are yellow, the new buds are orange-red in spring, the leaves are yellow-green in summer, and the leaves are golden-yellow in autumn. , has high horticultural ornamental value, the plant is mainly propagated by grafting at present, the survival rate is greatly affected by the environment, the survival rate is low, and the operation technology is difficult to master. There is also the problem of difficult reproduction of this new variety when it is propagated by tissue culture, that is, it must be In algebraic subculture, the leaves of test tube sprouts will have the problem of abscission and shedding, which will seriously affect further subculture

Method used

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  • Sterile micro grafting method of koelreuteria bipinnata var. intergrifoliola 'Jinyan'
  • Sterile micro grafting method of koelreuteria bipinnata var. intergrifoliola 'Jinyan'

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Effect test

Embodiment 1

[0013] The rootstock culture is to adopt excellent single-plant tissue culture of Huangshan eucalyptus. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75% Soak in alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. 3. The shoots of 4 leaves are used as rootstock grafting, and the medium is 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L+sucrose 30g / L; For scion culture, inoculate the buds of Jinyancailuan seedlings in mid-April to inoculate the tissue culture buds, and the medium is improved 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L + 30g / L sucrose, the cultivation time is 15-45 days, the dust on the surface of the material is cleaned before tissue culture, and then cut into 1-1.5cm stems with la...

Embodiment 2

[0015] The rootstock culture is to adopt excellent single-plant tissue culture of Huangshan eucalyptus. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75% Soak in alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. 3. The shoots of 4 leaves are used as rootstock grafting, and the medium is 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L+sucrose 30g / L; For scion culture, inoculate the buds of Jinyancailuan seedlings in mid-April to inoculate the tissue culture buds, and the medium is improved 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L + 30g / L sucrose, the cultivation time is 15-45 days, the dust on the surface of the material is cleaned before tissue culture, and then cut into 1-1.5cm stems with la...

Embodiment 3

[0017] The rootstock culture is to adopt excellent single-plant tissue culture of Huangshan eucalyptus. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75% Soak in alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. 3. The shoots of 4 leaves are used as rootstock grafting, and the medium is 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L+sucrose 30g / L; For scion culture, inoculate the buds of Jinyancailuan seedlings in mid-April to inoculate the tissue culture buds, and the medium is improved 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L + 30g / L sucrose, the cultivation time is 15-45 days, the dust on the surface of the material is cleaned before tissue culture, and then cut into 1-1.5cm stems with la...

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Abstract

The invention relates to a sterile micro grafting method of koelreuteria bipinnata var. intergrifoliola 'Jinyan'. The method mainly comprises the steps of stock culture, scion culture, stock selection, scion selection, test tube grafting and culture after grafting. Specifically, the good-liquid single-plant tissue culture bud shoot of the Huangshan goldenrain tree is taken as a stock and cultured for 15-45 days in a culture medium; the bud shoot obtained from the sowing seedling of koelreuteria bipinnata var. intergrifoliola 'Jinyan' by virtue of grafting and tissue culture is taken as a scion and cultured for 15-45 days in the culture medium; the test tube grafting method is a cleft grafting method, and the culture conditions after grafting are as follows: in an improved culture medium of 1 / 2MS+6-BA0.02mg / L+NAA(0.01-0.05)mg / L++IAA(0.03-0.08)mg / L+IBA(0.02-0.06)mg / L+cane sugar(20-40)g / L, illumination intensity is 2500lx, temperature condition is 25-27 DEG C, and illumination period is 12 hours; according to the grafting method, the survival rate is above 88% and the germination rate is above 88%.

Description

technical field [0001] The invention relates to a method for aseptic micro-grafting of eucalyptus with whole margins, in particular to a method for aseptic micro-grafting of eucalyptus japonica. Background technique [0002] Sterile micro-grafting, which is a combination of plant tissue culture and grafting technology. Micro-grafting, also known as test-tube grafting, is a technique of grafting rootstock and scion in a test tube to obtain new plants. It is based on tissue culture, and the grafted plants are cultivated under artificially controlled environmental conditions, eliminating the influence of external environmental factors on the survival of grafting. influences. [0003] Jinyan Cailuan (Koelreuteria bipinnata var. integrifoliola 'Jinyan') is an excellent variety selected from Huangshan eucalyptus. Its main characteristics are that the trunk or branches are yellow, the new buds are orange-red in spring, the leaves are yellow-green in summer, and the leaves are gold...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/06
CPCA01H4/00A01H4/001A01H4/005
Inventor 蒋泽平张敏黄利斌
Owner JIANGSU ACAD OF FORESTRY
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