A preparation method of black vinegar enzyme with hangover function
A vinegar enzyme and functional technology, applied in the field of enzymes, can solve the problems of pesticide pollution, affecting the function of enzymes, etc., and achieve the effects of stable process, improving the efficacy of traditional Chinese medicine, and eliminating toxic and side effects.
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Embodiment 1
[0046] Embodiment 1, the preparation method of the black vinegar enzyme that has hangover function of the present invention
[0047] (1) extract
[0048] Mix 1kg corn germ, 1kg Kansui, 1kg euphorbia, 1kg Chixiaodou, and 1kg horseshoe grass, grind them, add 70% ethanol 10 times the total dry weight of medicinal materials, reflux extraction twice, each time for 2 hours, filter, and combine the filtrates Concentrate and remove ethanol to obtain a traditional Chinese medicine extract, which is set aside, and the filter residue is dried at 60°C, pulverized, and set aside;
[0049] (2) Saccharification
[0050] a. Add water twice the weight of the filter residue to the filter residue obtained in step (1), use sodium citrate solution to adjust the pH value of the system to 5.0, add 0.8% of the filter residue weight of cellulase with an enzyme activity of 30000u / g, and heat at 50°C Under the shaking water bath for 3.0 h;
[0051] b. Adjust the temperature of the water bath to 92°C,...
Embodiment 2
[0065] Embodiment 2, the preparation method of the black vinegar enzyme that has hangover function of the present invention
[0066] (1) extract
[0067] Mix 1kg of corn germ, 1kg of kansui, 1kg of euphorbia, 1kg of red bean, and 1kg of horseshoe grass, grind them, add 70% ethanol that is 8 times the total weight of the medicinal materials, reflux extraction twice, each time for 2 hours, filter, combine the filtrates and Carry out concentration to remove ethanol, obtain the traditional Chinese medicine extract, set aside, filter residue is dried at 60 ℃, pulverized, set aside;
[0068] (2) Saccharification
[0069] a. Add water three times the weight of the filter residue to the filter residue obtained in step (1), use sodium citrate solution to adjust the pH of the system to 4.5, add 0.6% of the filter residue weight of cellulase with an enzyme activity of 30,000u / g, and heat at 50°C Shake the water bath for 3.5 h;
[0070] b. Adjust the temperature of the water bath to 90...
Embodiment 3
[0084] Embodiment 3, the preparation method of the black vinegar enzyme that has hangover function of the present invention
[0085] (1) extract
[0086] Mix 1kg corn germ, 1kg Kansui, 1kg euphorbia, 1kg Chixiaodou, and 1kg horseshoe grass, grind them, add 70% ethanol that is 9 times the total weight of medicinal materials, reflux extraction twice, each time for 3 hours, filter, combine the filtrates and Carry out concentration to remove ethanol, obtain the traditional Chinese medicine extract, set aside, filter residue is dried at 60 ℃, pulverized, set aside;
[0087] (2) Saccharification
[0088] a. Add water three times the weight of the filter residue to the filter residue obtained in step (1), use sodium citrate solution to adjust the pH of the system to 5.5, add 1.0% of the filter residue weight of cellulase with an enzyme activity of 30000u / g, and heat at 50°C Under the shaking water bath for 2.5 h;
[0089] b. Adjust the temperature of the water bath to 95°C, use so...
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