Molecular marker HRM5 of wheat few-tillering gene Ltn3 and application thereof

A technology of molecular markers and wheat, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of high selection difficulty, high cost, and long time, so as to improve the efficiency of selection and identification, The effect of high success rate and high accuracy

Inactive Publication Date: 2015-08-05
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Wheat yield traits are complex quantitative traits, which are controlled by multiple quantitative trait loci (Quantitative

Method used

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  • Molecular marker HRM5 of wheat few-tillering gene Ltn3 and application thereof
  • Molecular marker HRM5 of wheat few-tillering gene Ltn3 and application thereof
  • Molecular marker HRM5 of wheat few-tillering gene Ltn3 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0049] This embodiment is used to illustrate the acquisition method of wheat oligo-tiller gene Ltn3 and molecular marker HRM5:

[0050] (1) Using oligo-tiller wheat H461 as the female parent and wheat Chuannong 16 as the male parent to cross hybrids to obtain hybrid F1, F1 generation F2 was obtained by selfing a single plant, and the F8 generation RIL containing 223 lines was obtained by single seed propagation method 188 lines were randomly selected to form a genetic mapping population.

[0051] (2) Extract the DNA of each strain of the genetic mapping population with the CTAB method, and select the hexaploid wheat A, B, D published on wheatgenomics (http: / / wheatgenomics.plantpath.ksu.edu / ) The 90K SNP chip of the genome, using the DNA of the parents H461 and Chuannong 16 as templates, was used for genotyping to obtain the genotype data of the RIL population. The band pattern of the parent H461 is marked as A, and the band pattern of the parent Chuannong 16 is marked as B. ...

Embodiment 2

[0061] 1.1 Extraction of DNA

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Abstract

The invention discloses a molecular marker HRM5 which is closely linked with wheat few-tillering gene Ltn3, wherein the nucleotide sequence of the molecular marker HRM5 is represented as the SEQ ID No.1. Genetic distance between the molecular marker and the wheat few-tillering gene Ltn3 is 0.35 cM. A test result proves that the molecular marker can accurately trace the wheat few-tillering gene and predict tillering characters of wheat, thereby further carrying molecular-designing breeding conveniently. The invention also discloses a method of identifying the molecular marker of the wheat few-tillering gene Ltn3. By means of the method, accuracy of tillering prediction can be increased, success rate of specific plant type breeding can be increased, and achievement of an object of increasing per unit area yield of wheat is accelerated.

Description

technical field [0001] The invention relates to the field of wheat molecular breeding, in particular to a molecular marker HRM5 of wheat oligo-tiller gene Ltn3 and an application thereof. Background technique [0002] Wheat is the second largest grain crop after rice in my country. The planting area over the years accounts for 22% to 30% of the cultivated land area and 22% to 27% of the total grain crop area. It is mainly distributed in Henan, Hebei, Shandong, Shanxi, Shaanxi, Jiangsu, Sichuan, Anhui and other provinces; the total output is more than 100 million tons, accounting for about 22% of the grain crop output, and it is the staple food of about half of the population in my country. Therefore, the breeding of high-yielding wheat varieties has always been the focus of breeders. [0003] Wheat yield traits are complex quantitative traits, which are controlled by multiple quantitative trait loci (Quantitative trait locus, QTL). The traditional breeding method has the pro...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 刘亚西王智强高尚石浩然莫洪君卢艳丽王益魏育明郑有良
Owner SICHUAN AGRI UNIV
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