Device for monitoring spatial coagulation of blood and of components thereof
A space and blood technology, applied in the fields of biotechnology and basic biological research, can solve the problems of hindering the analysis of blood fibrous clots, hindering the temporal and spatial distribution of proteolytic enzymes, and distorting the light scattering signal of blood fibrous clots, etc. The effect of reliability
Active Publication Date: 2015-08-05
血液有限责任公司
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Problems solved by technology
[0004] Disadvantages of the device include the generation of air bubbles in the test sample and fluid in the recording area during heating of the test sample in the thermostatically controlled chamber, which distort the light scattering signal from the fibrous clot
In addition, the device contains an illumination device with only one wavelength, for example red light, which prevents the analysis of the spatiotemporal distribution of proteolytic enzymes using, for example, fluorescent methods and at the same time the analysis of the formation of blood clots
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[0013] The device of the invention comprises a thermostatically controlled chamber 1 arranged to allow temperature regulation and filled with a fluid. Various types of thermostatic control can be provided, including thermostatic control of water and thermostatic control of air as well as those involving the use of gels; additionally, it needs to be taken into account that the test medium must remain transparent for irradiation. Water thermostatic control is preferred. During thermostatic control, a constant temperature is maintained. The cuvette 2 is placed inside the constant temperature control chamber 1 . The cuvette 2 can be provided with at least one channel 3 . A sample of the test medium is placed in channel 3. The test medium sample may represent a mammalian (human or animal) biological fluid such as plasma, whole blood, platelet poor plasma or platelet rich plasma. Furthermore, the sample may contain a mixture of purified natural, synthetic or recombinant proteins...
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The present invention relates to medicine and biology and can be used, in particular, for diagnostic and research purposes to determine coagulation characteristics of blood and its components, as well as in biotechnology, and in fundamental biological research. The object of this invention is to exclude the influence of gas bubbles in the test sample and in the thermostatically controlled fluid on the test process itself (for example, on blood coagulation) and on processing of the registered data, while it has an impact on the test integrity and on accuracy of the obtained results as well as on acquisition of new information on coagulation process and specific parameters thereof. The object is solved by creating a device for monitoring of spatial coagulation of blood and its components comprising: a thermostatically controlled chamber, at least one means of illumination, a means of recording and a means of pressure regulation connected with the thermostatically controlled chamber, wherein the thermostatically controlled chamber includes a cuvette to place a sample of a test medium, a light trap, and is filled with a substance suitable for temperature regulation, and wherein the light trap is formed by geometry of the inner surfaces of the thermostatically controlled chamber, and with a pressure-control means which is connected to the temperature-controlled chamber or to the cuvette.
Description
technical field [0001] The technical solution relates to medicine and biology and can be used for determining the coagulation properties of blood and its components, inter alia for diagnostic and research purposes, as well as for biotechnological and basic biological research. Background technique [0002] Coagulation analysis of blood and its components is of great practical importance, since it not only allows the diagnosis of certain diseases, but also allows the assessment of the activity of agents affecting blood coagulation parameters. Various devices for determining the coagulation rate in whole blood as well as in plasma are known in the prior art. However, until now, these assays have been designed in homogeneous systems utilizing permanent mixing. Since the full volume of plasma is permanently mixed from the beginning of the test until the end of the test, all coagulation factors formed during the process of forming a plasma clot are evenly distributed in the test...
Claims
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IPC IPC(8): G01N33/49
CPCB01L2300/14G01N21/82G01N33/49G01N21/0332B01L2300/185G01N2201/0642B01L99/00C12Q1/56
Inventor F·I·阿塔乌拉克哈诺夫V·I·萨巴斯M·A·潘特里夫S·S·卡拉姆津
Owner 血液有限责任公司