Type-A foot-and-mouth disease (FMD) marking vaccine and construction method thereof

Abstract

The invention discloses a type-A foot-and-mouth disease (FMD) marking vaccine and a construction method thereof. The seed virus of the disclosed marking vaccine is an A / rV-2 virus with a gene fragment as shown in SEQ ID NO:3; the amino acid sequence encoded according to the gene fragment is as shown in a sequence table as follows: SEQ ID NO:4; and the amino acid sequence is obtained by deleting amino acids from the 104th position to 115th position of a type-3A FMD virus protein. An animal infected by the FMD marking virus constructed by using the construction method is remarkably different from an animal infected by a wild-type virus on the aspect of serology; and an FMD marking vaccine immunized pig has favorable immunogenicity. Therefore, the type-A FMD virus constructed by using the method disclosed by the invention can be used for developing a differential diagnosis (DIVA) vaccine which is used for preventing and controlling the current type-A FMD in China.

Description

technical field [0001] The invention relates to a type A foot-and-mouth disease labeled vaccine and a construction method thereof, belonging to the fields of animal medicine and immunobiology. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile and highly contagious infectious disease infecting cloven-hoofed animals such as pigs, cattle and sheep. The disease is highly contagious, and its outbreak and prevalence will bring huge losses to animal husbandry and related industries. It is listed as the first disease that must be reported by the World Organization for Animal Health (FAO / OIE), and is defined as a first-class animal infectious disease in my country. [0003] The pathogen of FMD is foot-and-mouth disease virus (FMDV), which has 7 different serotypes (A, O, C, AsiaI, SAT I, SAT II and SAT III) and many subtypes, and there is no cross protection between types. my country is the hardest-hit country of FMD, O-type FMD has been prevalent for a...

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Problems solved by technology

In response to this demand, Fowler VL et al. developed a marked vaccine by deleting the G-H loop of the FMDV structural protein. Practical applications showed that although this marked vaccine can completely protect cattle against strong virus attacks and achieve the purpose of differential diagnosis, the G-H loop of FMDV and It is the main neutralizing antigenic site, and the deletion of this region will lead to a decrease in the immunogenicity of the vaccine and affect the immune effect

Uddowla S et al. developed FMDV 3B protein deletion and / or 3D protein modified A-type attenuated labeled vaccines. Practical applications have shown that this type of labeled vaccine can meet the needs of immune protection and differential diagnosis, but excessive attenuation of the vaccine cannot induce good protection. immune response

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