Type-A foot-and-mouth disease (FMD) marking vaccine and construction method thereof

A technology of foot-and-mouth disease and foot-and-mouth disease virus, which is applied in the field of animal medicine and immunobiology, can solve the problems of excessive weakening of vaccines, reduction of vaccine immunogenicity, inability to induce protective immune responses, etc., and achieve good immunogenicity effects

Active Publication Date: 2015-08-12
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In response to this demand, Fowler VL et al. developed a marked vaccine by deleting the G-H loop of the FMDV structural protein. Practical applications showed that although this marked vaccine can completely protect cattle against strong virus attacks and achieve the purpose of differential diagnosis, the G-H loop of FMDV and It is the main neutralizing antigenic site, and the deletion of this region will lead to

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Type-A foot-and-mouth disease (FMD) marking vaccine and construction method thereof
  • Type-A foot-and-mouth disease (FMD) marking vaccine and construction method thereof
  • Type-A foot-and-mouth disease (FMD) marking vaccine and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The present invention will be further described below in conjunction with the examples, and the experimental methods used in the following examples are conventional methods unless otherwise specified.

[0031] The implementation process of the present invention is realized according to the following steps:

[0032] 1. Construction of genome full-length cDNA clone

[0033] According to the published full genome sequence of type A FMDV, the A / GDMM / CHA / 2013 strain (GenBank: KF450794.1) genome is divided into 4 overlapping segments (the overlap referred to here refers to the end of the last gene segment and the The beginning of the next gene fragment has dozens to hundreds of nucleotide overlaps, and the selection of specific overlapping regions is not particularly limited), and 4 pairs of oligonucleotide primers are designed and synthesized, and the sequences of the primers are A1 ( +): SEQ ID NO: 5, A1(-): SEQ ID NO: 6; A2(+): SEQ ID NO: 7, A2(+): SEQ ID NO: 8; A3(+): SE...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a type-A foot-and-mouth disease (FMD) marking vaccine and a construction method thereof. The seed virus of the disclosed marking vaccine is an A/rV-2 virus with a gene fragment as shown in SEQ ID NO:3; the amino acid sequence encoded according to the gene fragment is as shown in a sequence table as follows: SEQ ID NO:4; and the amino acid sequence is obtained by deleting amino acids from the 104th position to 115th position of a type-3A FMD virus protein. An animal infected by the FMD marking virus constructed by using the construction method is remarkably different from an animal infected by a wild-type virus on the aspect of serology; and an FMD marking vaccine immunized pig has favorable immunogenicity. Therefore, the type-A FMD virus constructed by using the method disclosed by the invention can be used for developing a differential diagnosis (DIVA) vaccine which is used for preventing and controlling the current type-A FMD in China.

Description

technical field [0001] The invention relates to a type A foot-and-mouth disease labeled vaccine and a construction method thereof, belonging to the fields of animal medicine and immunobiology. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile and highly contagious infectious disease infecting cloven-hoofed animals such as pigs, cattle and sheep. The disease is highly contagious, and its outbreak and prevalence will bring huge losses to animal husbandry and related industries. It is listed as the first disease that must be reported by the World Organization for Animal Health (FAO / OIE), and is defined as a first-class animal infectious disease in my country. [0003] The pathogen of FMD is foot-and-mouth disease virus (FMDV), which has 7 different serotypes (A, O, C, AsiaI, SAT I, SAT II and SAT III) and many subtypes, and there is no cross protection between types. my country is the hardest-hit country of FMD, O-type FMD has been prevalent for a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/135A61K48/00A61P31/14C12N15/86C12N15/42
Inventor 李平花刘在新孙普袁红白兴文卢曾军李冬陈应理包慧芳付元芳曹轶梅
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap