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Preservative for preserving free DNA in peripheral blood

A preservative and peripheral blood technology, applied in the field of DNA preservation, can solve the problems of short storage time and low storage temperature

Inactive Publication Date: 2015-08-12
厦门万基生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a preservative for preserving free DNA in peripheral blood, aiming to solve the problems of short storage time and low storage temperature in the existing plasma free DNA storage methods

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  • Preservative for preserving free DNA in peripheral blood
  • Preservative for preserving free DNA in peripheral blood
  • Preservative for preserving free DNA in peripheral blood

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Embodiment Construction

[0030] The present invention provides a preservative for preserving free DNA in peripheral blood. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0031] A kind of preservative for preserving free DNA in peripheral blood provided by the present invention, by mass percentage, comprises:

[0032] Ultrapure sterile water 60~80%;

[0033] Anticoagulant 5~8%;

[0034] Preservatives 10~25%;

[0035] Metabolic inhibitor 1~5%

[0036] Nuclease inhibitor 0.5~3%.

[0037] Among them, the ultrapure sterile water is sterilized ultrapure water.

[0038] Further, the anticoagulant includes one or more combinations of EDTA potassium salt, EDTA sodium salt, sodium citrate, heparin, and sodium wolfberry. The main func...

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Abstract

The invention discloses a preservative for preserving free DNA in peripheral blood. The preservative is composed of the following components in percentage by weight: 60 to 80% of ultrapure sterile water, 5 to 8% of anti-coagulant, 10 to 25% of antiseptic, 1 to 5% of metabolic inhibitor, and 0.5 to 3% of nuclease inhibitor. The provided preservative can preserve peripheral blood for at least 7 days at a normal temperature, 14 days at most, the blood can be transported at a normal temperature for 72 hours or more under the assistance of the preservative, 96 hours at most; and the free DNA amount is basically not affected during the period.

Description

technical field [0001] The invention relates to the field of DNA preservation, in particular to a preservative for preserving free DNA in peripheral blood. Background technique [0002] At present, there are more and more clinical applications of cell-free DNA in peripheral blood. For example, non-invasive prenatal genetic testing and tumor diagnosis both use cell-free DNA in peripheral blood as the detection target. The amount of free DNA is a key indicator during the detection process. If there are white blood cells or nucleated red blood cells in the peripheral blood, the genomic DNA will degrade and enter the plasma to contaminate the free DNA, which will seriously affect the test results. Therefore, in the above testing process, the peripheral blood is required to separate the plasma within 6 hours, store and transport at low temperature, and the storage time should not be too long. [0003] Therefore, the prior art still needs to be improved and developed. Contents ...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 陈洪亮段利朋郑海灵
Owner 厦门万基生物科技有限公司
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