Preservative for preserving free DNA in peripheral blood

Abstract

The invention discloses a preservative for preserving free DNA in peripheral blood. The preservative is composed of the following components in percentage by weight: 60 to 80% of ultrapure sterile water, 5 to 8% of anti-coagulant, 10 to 25% of antiseptic, 1 to 5% of metabolic inhibitor, and 0.5 to 3% of nuclease inhibitor. The provided preservative can preserve peripheral blood for at least 7 days at a normal temperature, 14 days at most, the blood can be transported at a normal temperature for 72 hours or more under the assistance of the preservative, 96 hours at most; and the free DNA amount is basically not affected during the period.

Description

technical field [0001] The invention relates to the field of DNA preservation, in particular to a preservative for preserving free DNA in peripheral blood. Background technique [0002] At present, there are more and more clinical applications of cell-free DNA in peripheral blood. For example, non-invasive prenatal genetic testing and tumor diagnosis both use cell-free DNA in peripheral blood as the detection target. The amount of free DNA is a key indicator during the detection process. If there are white blood cells or nucleated red blood cells in the peripheral blood, the genomic DNA will degrade and enter the plasma to contaminate the free DNA, which will seriously affect the test results. Therefore, in the above testing process, the peripheral blood is required to separate the plasma within 6 hours, store and transport at low temperature, and the storage time should not be too long. [0003] Therefore, the prior art still needs to be improved and developed. Contents ...

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a preservative for preserving fre

Images