Marine sediment organic pollution rapid monitoring method

Abstract

The present invention provides a marine sediment organic pollution rapid monitoring method which mainly includes the following steps: extracting sediment environment total DNA; using a primer for amplification of 16 s rDNA fragments; cloning and determining DNA sequence; comparing the obtained DNA sequence with Candidate division WS3 16 s DNA sequence (SEQ ID NO. 3) and Tenericutes 16 s DNA sequence (SEQ ID NO. 4); and analyzing Candidate division WS3 and Tenericutes microorganisms exist and content. The marine sediment organic pollution rapid monitoring method combines the advantages of the molecular biological technique and indicator biological method, organic pollutants (organochlorine pesticides and polychlorinated biphenyls (PCBS)) and indicator microorganisms (Candidate division WS3 and Tenericutes) of the organic pollutants are directly corresponded, marine complex organic matter polluted environment fast and exact monitoring can be achieved, key pollutant kind of comprehensive organic matter polluted environment can be identified, and the influence of pollution comprehensive effect on microbial community and the ecological system can be predicted.

Description

technical field [0001] The invention relates to the field of environmental monitoring, in particular to a method for rapid monitoring of organic pollution of marine sediments based on the 16S gene fragment of marine microorganisms. Background technique [0002] Organic pollutants refer to pollutants composed of natural organic substances in the form of carbohydrates, proteins, amino acids and fats, and some other biodegradable synthetic organic substances, which can be divided into natural organic pollutants and synthetic organic pollutants. Two types of pollutants. Organic pollutants have the characteristics of high toxicity, persistence and bioaccumulation effect. The widespread organic pollution in nature has become one of the important environmental problems that human beings need to solve urgently. Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) are highly toxic persistent organic pollutants that can exist in the environment for a long time and ac...

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Problems solved by technology

Physical and chemical testing can only reflect the partial and instantaneous state of marine pollution, but cannot reflect the overall pollution effect of the ocean

At the same time, physical and chemical monitoring can only detect the content and type of various pollutants. If unknown pollutant pollution, biological pollution or large-scale mixed pollution occurs, there will be both synergistic and antagonistic effects between various pollutants, and additive effects, etc., which cannot accurately assess the degree of pollution of the marine environment

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