Marine sediment organic pollution rapid monitoring method

A technology for marine sediments and organic pollutants, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms.

Inactive Publication Date: 2015-08-12
浙江省舟山海洋生态环境监测站 +1
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Problems solved by technology

Physical and chemical testing can only reflect the partial and instantaneous state of marine pollution, but cannot reflect the overall pollution effect of the ocean
At the same time, physical and chemical monitoring can only detect the content and type of various pollutants. If u

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  • Marine sediment organic pollution rapid monitoring method
  • Marine sediment organic pollution rapid monitoring method
  • Marine sediment organic pollution rapid monitoring method

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Embodiment 1

[0024] In the present embodiment, (1) organic pollutant is measured: sediment organochlorine, polychlorinated biphenyl concentration all refer to the organochlorine pesticide and polychlorinated biphenyl compound standard method (USEPA 8270D- 2007) determination. (2) Determination of microbial 16S sequence: extract the total DNA from the sediment environment, and perform PCR amplification with 27F and 1492R primers; send it to Shanghai Meiji Biotechnology Company for sequence determination. The obtained sequences were searched in the American NCBI GenBank database, and microbial species were identified, including Candidate division WS3, Tenericutes, etc. (3) Correlation analysis: Apply the Spearman rank correlation analysis method to conduct correlation analysis on the concentration of various environmental parameters, microbial species and sequence numbers in the sediment, and calculate the correlation coefficient and significance between each environmental parameter and each...

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Abstract

The present invention provides a marine sediment organic pollution rapid monitoring method which mainly includes the following steps: extracting sediment environment total DNA; using a primer for amplification of 16 s rDNA fragments; cloning and determining DNA sequence; comparing the obtained DNA sequence with Candidate division WS3 16 s DNA sequence (SEQ ID NO. 3) and Tenericutes 16 s DNA sequence (SEQ ID NO. 4); and analyzing Candidate division WS3 and Tenericutes microorganisms exist and content. The marine sediment organic pollution rapid monitoring method combines the advantages of the molecular biological technique and indicator biological method, organic pollutants (organochlorine pesticides and polychlorinated biphenyls (PCBS)) and indicator microorganisms (Candidate division WS3 and Tenericutes) of the organic pollutants are directly corresponded, marine complex organic matter polluted environment fast and exact monitoring can be achieved, key pollutant kind of comprehensive organic matter polluted environment can be identified, and the influence of pollution comprehensive effect on microbial community and the ecological system can be predicted.

Description

technical field [0001] The invention relates to the field of environmental monitoring, in particular to a method for rapid monitoring of organic pollution of marine sediments based on the 16S gene fragment of marine microorganisms. Background technique [0002] Organic pollutants refer to pollutants composed of natural organic substances in the form of carbohydrates, proteins, amino acids and fats, and some other biodegradable synthetic organic substances, which can be divided into natural organic pollutants and synthetic organic pollutants. Two types of pollutants. Organic pollutants have the characteristics of high toxicity, persistence and bioaccumulation effect. The widespread organic pollution in nature has become one of the important environmental problems that human beings need to solve urgently. Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) are highly toxic persistent organic pollutants that can exist in the environment for a long time and ac...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/06C12Q1/04
CPCC12Q1/689
Inventor 黄备何劲儒魏娜
Owner 浙江省舟山海洋生态环境监测站
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