A micro ecological feed additive for regulating rumens and a preparation method thereof

A micro-ecological feed and rumen regulation technology, which is applied in the field of microbial fermentation, can solve the problems of difficult long-term preservation of live bacteria preparations, weak resistance to inactivation, and difficulty in colonization, so as to improve animal production performance, broaden the scope of use, and reduce impurities. The effect of bacterial contamination

Inactive Publication Date: 2015-08-19
CHANGCHUN BORUI FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Avoid live bacteria preparations that are not easy to store for a long time, not resistant to high temperature, activity gradually decreases with storage time, difficult to colonize in animal intestines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the rumen-regulated micro-ecological feed additive comprises the following steps:

[0032] Step 1. Preparation of yeast metabolites and intracellular substances: (1) Inoculate Saccharomyces cerevisiae strains into YPD slant medium, and culture them statically at 28°C for 48 hours to obtain revived Saccharomyces cerevisiae strains; (2) Inoculate the revived Saccharomyces cerevisiae strains Saccharomyces cerevisiae strains were inoculated into the liquid YPD medium, and cultivated to obtain the first-grade seed liquid; (3) inoculated the first-grade seed liquid into the aerobic fermentation medium in an amount of 4%, and fermented aerobically at 30° C. for 24 hours to obtain the second-grade seed liquid. Seed liquid; (4) liquid anaerobic fermentation: inoculate the secondary seed liquid into liquid anaerobic fermentation medium with an inoculum size of 20%, and anaerobically ferment at 15°C for 24 hours to obtain anaerobic fermentation product; (5...

Embodiment 2

[0038] The preparation method of the rumen-regulated micro-ecological feed additive comprises the following steps:

[0039] Step 1, preparation of yeast metabolites and intracellular substances: same as Step 1 of Example 1;

[0040] Step 2, the preparation of aspergillus oryzae metabolite: with the step 2 of embodiment 1;

[0041] Step 3. Preparation of the rumen-regulated microecological feed additive: uniformly mix the yeast metabolites and intracellular substances in the step 1 and the Aspergillus oryzae metabolites in the step 2 at a mass ratio of 15:1 to obtain the rumen-regulated microecological feed additive.

Embodiment 3

[0043] The preparation method of the rumen-regulated micro-ecological feed additive comprises the following steps:

[0044] Step 1, preparation of yeast metabolites and intracellular substances: same as Step 1 of Example 1;

[0045] Step 2, the preparation of aspergillus oryzae metabolite: with the step 2 of embodiment 1;

[0046] Step 3, preparation of the rumen-regulated micro-ecological feed additive: uniformly mix the yeast metabolites and intracellular substances in the step 1 and the Aspergillus oryzae metabolites in the step 2 at a mass ratio of 25:1 to obtain the rumen-regulated micro-ecological feed additive.

[0047] Product efficacy verification

[0048] 1. Experimental animals

[0049] Select 4 sheep (18 months old) in good body condition and install permanent rumen fistula for the collection of rumen fluid. The fine-grain ratio is set at 40:60, and the green hay (Leymus chinensis) is free to eat; feed twice a day at 8:00 am and 17:00 am, respectively, and add c...

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Abstract

The present invention belongs to the field of microbial fermentation technology, and particularly to a micro ecological feed additive for regulating rumens and a preparation method thereof. The additive consists of metabolites and intracellular substances of yeast, and metabolites of aspergillus oryzae with a mass ratio of 15: 1, 20: 1 or 25: 1. Single strain industrial fermentation technology is used to produce a specific anti-stress metabolite composite, the method is stable in process, and effectively solves the defects that viable organism and enzyme preparations are easily inactivated and unstable, which greatly expands the application range of the additive, and is conducive to achieve the targeted product development of different animals and different physiological stages of the animals. The additive is not a viable organism preparation, but is the specific anti-stress metabolite composite produced by viable organisms after high density fermentation, and the additive is easy to store so that active ingredient activity cannot be decreased with prolonged storage time, which ensures active ingredient contents of the additive.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a rumen-regulated micro-ecological feed additive and a preparation method thereof. Background technique [0002] Microecological preparations refer to the normal flora and their metabolites and the material preparations that selectively promote the growth of normal flora of the host under the guidance of the theory of microecology to adjust the imbalance of microecological balance, maintain the balance of microecology, and improve the health of the host. general term. The main functions of microecological feed additives are: to adjust the microecological balance in the intestinal tract of animals, increase the growth rate of animals, improve the utilization rate of feed, and promote the absorption of calcium, magnesium and iron by animals; inhibit the reproduction of harmful microorganisms in the intestinal tract, and improve the health level of animals...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/14C12N1/06C12R1/865C12R1/69
Inventor 甄玉国赵小丽张学锋赵巍郑艳秋关艳玲
Owner CHANGCHUN BORUI FEED
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