Tongling peony tissue culture and rapid propagation seedling raising technical method

Abstract

The invention discloses a Tongling peony tissue culture and rapid propagation seedling raising technical method. The Tongling peony tissue culture and rapid propagation seedling raising technical method comprises the following steps of explant selection which comprises selecting Tongling peony buds as an explant; explant disinfection which comprises stripping scales after the explant is washed through the flowing water until the explant is clear, performing 30 seconds of soaking through 70% of volume fraction of alcohol, performing 1 minute of sterilization through 0.1% of concentration of mercuric chloride and performing three times of rinsing through sterile water, wherein every time of rinsing lasts 1 minute; culture medium induction through cluster buds, wherein the formula is as follows and the culture period is 15 days; culture medium rooting, wherein the formula is as follows and the culture period is 10 to 15 days; acclimatization and transplanting which comprise appropriately opening a culture bottle cap for the gas exchange inside and outside a bottle, completely opening the culture bottle cap after one day, taking out seedlings after two days of acclimatization, washing a culture medium on the seedling base portion and root in the warm water, transplanting the seedlings into a greenhouse and covering a plastic film for moisturizing, wherein 400 times of carbendazim treatment is performed on matrixes before transplanting, 800 times of carbendazim is sprayed once every week after transplanting, the temperature is 20 plus or minus 2 DEG C, and the humidity is 85%; culture period settings which comprise setting the first time of culture period as a month and shortening the culture period into half a month after a tissue culture system is built.

Description

technical field [0001] The invention relates to a technical method of biological agriculture, in particular to a technical method of tissue culture and rapid propagation of fengdan. Background technique [0002] Tissue culture technology is to place living organs, tissues or cells in the culture medium under sterile conditions and place them in a suitable environment for continuous culture of cells, tissues or individuals. This technology has been widely used Agricultural and biological, medical research. However, the technology and medium formula for using the rapid propagation technology in the production of Fengdan seedlings have not been found, which is very much needed in practical applications. Contents of the invention [0003] The purpose of the present invention is to overcome the deficiencies in the prior art, and provide a technical method for the rapid propagation of the phoenix dan group for cultivating seedlings of the phoenix dan group. [0004] The presen...

AI Technical Summary

Problems solved by technology

However, the technology and medium formula for using the rapid propagation technology in the production of Fengdan seedlings have not yet been found, which is very much needed in practical applications.