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Chemical grafted type long-acting sustained-release silk suture with antibacterial function and preparation method of silk suture

A chemical grafting and suture technology, applied in medical science, surgery, surgical instruments, etc., can solve problems such as increasing surgical site infection, and achieve excellent biocompatibility and good biocompatibility.

Inactive Publication Date: 2015-08-26
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since silk fibroin is a protein fiber, it is easy to become a nutrient source for the growth and reproduction of pathogenic bacteria; at the same time, the braided structure makes there are gaps between the weaving yarns inside the suture, which provides physical conditions for pathogenic bacteria to adhere and colonize
Each of these conditions increases the risk of postoperative surgical site infections (SSIs)

Method used

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  • Chemical grafted type long-acting sustained-release silk suture with antibacterial function and preparation method of silk suture
  • Chemical grafted type long-acting sustained-release silk suture with antibacterial function and preparation method of silk suture
  • Chemical grafted type long-acting sustained-release silk suture with antibacterial function and preparation method of silk suture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] a) Preparation of hydrogel monomer

[0043] (1) Accurately weigh 60.405 g of adipoyl chloride, 110 g of p-nitrophenol, 71.88 g of triethylamine, and 600 mL of acetone. Add p-nitrophenol, triethylamine and 450mL acetone successively to a 1L round bottom flask, and mix thoroughly with a magnetic stirrer at room temperature. Stir the mixed solution with magnetic force at -78°C, add the adipoyl chloride solution dissolved in 150 mL of acetone drop by drop using a constant pressure dropping funnel, and fully react for 4-6 hours. After the reaction is complete, the product is repeatedly washed by suction filtration with pure water. When the impurities in the product are completely removed, the product is further vacuum-dried to remove the moisture contained in the product and the p-nitrophenyl adipate is collected and stored.

[0044] (2) Accurately weigh 85.12g of lysine hydrochloride and 20g of 1,4-butanediol and mix them into a 1L single-layer glass reactor, add an approp...

Embodiment 2

[0053] a) Preparation of hydrogel monomer

[0054] The preparation steps of hydrogel monomer are identical with example 1;

[0055] b) Preparation of hydrogels with antibacterial properties

[0056] Weigh p-nitrophenyl adipate and lysine monomer according to the molar ratio of 2:1 and add the monomer into N,N-dimethylacetamide (DMAc) solution to make the concentration of lysine monomer 0.5g / mL mixed solution. At 50°C until the monomer is completely dissolved, then add 1.5 mL of triethylamine solution with an antibacterial agent concentration of 5000 μg / mL and continue to react for 1 hour at 80°C. After complete reaction, the synthesized drug-loaded hydrogel was washed repeatedly with pure water to remove residual chemical substances in the hydrogel.

[0057] c) Preparation of antibacterial nanoparticles

[0058] Trypsin was dissolved in PBS buffer to make a 0.1 mg / mL degradation solution, and the nanoparticle solution was obtained after degrading the hydrogel for one week ...

Embodiment 3

[0063] a) Preparation of hydrogel monomer

[0064] The preparation steps of hydrogel monomer are identical with example 1;

[0065] b) Preparation of hydrogels with antibacterial properties

[0066] Weigh p-nitrophenyl adipate and lysine monomer according to the molar ratio of 2:1 and add the monomer into N,N-dimethylacetamide (DMAc) solution to make the concentration of lysine monomer 0.5g / mL mixed solution. At 50°C until the monomers are completely dissolved, then add 1.5 mL of triethylamine solution with an antibacterial agent concentration of 6500 μg / mL and continue to react for 1 hour at 80°C. After complete reaction, the synthesized drug-loaded hydrogel was washed repeatedly with pure water to remove residual chemical substances in the hydrogel.

[0067] c) Preparation of antibacterial nanoparticles

[0068] Trypsin was dissolved in PBS buffer to make a 0.1 mg / mL degradation solution, and the nanoparticle solution was obtained after degrading the hydrogel for one wee...

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Abstract

The invention relates to a chemical grafted type long-acting sustained-release silk suture with an antibacterial function and a preparation method of the silk suture. The chemical grafted type long-acting sustained-release silk suture comprises braiding yarn and nanoparticles with the antibacterial function. The silk suture is activated in a buffer solution containing EDC and NHS, the nanoparticles with the antibacterial function are added for grafting, and the chemical grafted type long-acting sustained-release silk suture is obtained. The silk suture contains an antibacterial agent on the surface, thus having sterilizing and bacterial inhibition functions; the antibacterial agent is uniformly dispersed in the nanoparticles and antibacterial drugs are continuously and slowly released with the continuous biodegradation of nanoparticles, so that the silk suture has long-acting and stable sustained release and antibacterial functions.

Description

technical field [0001] The invention belongs to the field of functional silk suture and its preparation, in particular to a chemically grafted long-acting slow-release antibacterial silk suture and a preparation method thereof. Background technique [0002] With the development of medical scientific research technology and the summarization of experience in clinical practice, although systematic antibiotic preventive measures have been established, surgical site infections (SSIs) are still a very challenging complication. It has been reported that the incidence of surgical site infections (SSIs) is generally 2-5%, but for example in colorectal surgery the infection rate can rise to 25%. Once the wound is infected, the injured generally need up to 10 days of delayed hospital treatment. Therefore, infection of the surgical site means more expensive treatment consumption and loss of excessive medical resources. In recent years, it has been clinically confirmed that surgical s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L17/14A61L17/06
Inventor 吴德群崔海春
Owner DONGHUA UNIV
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