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Tobacco stem microbiological treatment aroma increasing method

A technology for microbial treatment and aroma extraction, which is applied in the field of tobacco stem treatment for aroma and acidophilus, can solve the problems of astringency, limit the proportion of cut stems, and the weight of miscellaneous gas, so as to achieve excellent aroma extraction effect and improve utilization. , easy to produce effect

Active Publication Date: 2015-09-16
江苏浩丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pyrolysis products of this kind of substances are highly irritating, heavy miscellaneous gas, coughing, astringent, and less aroma, which seriously affects the smoking quality of cigarettes and directly limits the proportion of shredded stems in the leaf formula.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for microbial treatment of tobacco stems to enhance aroma, characterized in that the steps are as follows:

[0021] Get Micrococcus acidophilus and Bacillus subtilis to inoculate in an additional 200ml LB liquid culture medium containing 0.2% trehalose by weight according to the inoculum size of 0.1% and 0.15% respectively;

[0022] Shake at 37°C at 160r / min to OD value = 2.2; centrifuge at 3800rpm / min for 8min, then wash the precipitate with sterile water, centrifuge, shake evenly with 20mL of sterile water, and dilute 15% when used. times, and adding pectinase is the bacterial agent for treatment;

[0023] Weigh the cut stems, spray 0.12 times the weight of the cut stems on the cut stems, seal it, and keep it at a constant temperature for 5 hours at 30°C, then dry the cut stems, and then put the cut stems at a constant temperature of 20°C and a relative humidity of 50%. Equilibrate in a constant humidity chamber for 48 hours.

[0024] Prepare each liter of ...

Embodiment 2

[0030] A method for microbial treatment of tobacco stems to enhance aroma, characterized in that the steps are as follows:

[0031] Get Micrococcus acidophilus, and Bacillus subtilis is inoculated in 200ml of LB liquid medium that contains 0.2% trehalose by weight percentage according to the inoculum size of 0.1% and 0.15% respectively;

[0032] Shake at 37°C at 160r / min to OD value = 2.2; centrifuge at 3800rpm / min for 8min, then wash the precipitate with sterile water, centrifuge, shake evenly with 20mL of sterile water, and dilute 15% when used. times, and adding pectinase is the bacterial agent for treatment;

[0033] Weigh the cut stems, spray 0.15 times the weight of the cut stems on the cut stems, seal it, and keep it at a constant temperature for 5 hours at 30°C, then dry the cut stems, and put the cut stems at a constant temperature of 22°C and a relative humidity of 60%. Equilibrate in a constant humidity chamber for 48 hours.

[0034] Prepare each liter of LB mediu...

Embodiment 3

[0040] A method for microbial treatment of tobacco stems to enhance aroma, characterized in that the steps are as follows:

[0041] Get Micrococcus acidophilus, and Bacillus subtilis is inoculated in 200ml of LB liquid medium that contains 0.2% trehalose by weight percentage according to the inoculum size of 0.1% and 0.15% respectively;

[0042] Shake at 37°C at 160r / min to OD value = 2.2; centrifuge at 3800rpm / min for 8min, then wash the precipitate with sterile water, centrifuge, shake evenly with 20mL of sterile water, and dilute 15% when used. times, and adding pectinase is the bacterial agent for treatment;

[0043] Weigh the cut stems, spray 0.13 times the weight of the cut stems on the cut stems, seal it, and keep it at a constant temperature for 5 hours at 30°C, then dry the cut stems, and put the cut stems at a constant temperature of 21°C and a relative humidity of 55%. Equilibrate in a constant humidity chamber for 48 hours.

[0044] Prepare each liter of LB mediu...

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PUM

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Abstract

The invention discloses a tobacco stem microbiological treatment aroma increasing method. The tobacco stem microbiological treatment aroma increasing method is characterized by comprising the following steps that pediococcusacidilactici and bacillus subtilis are taken and inoculated in an LB fluid medium containing, by weight, 0.2% of trehalose; centrifugalization is carried out after culture, then sterile water is used for washing and settling, centrifugalization is carried out, a product is evenly oscillated with sterile water, and the product is diluted into a microbial agent for treatment when used; the microbial agent is simply taken and sprayed on cut stems to carry out treatment.

Description

technical field [0001] The invention belongs to the technical field of tobacco additives, in particular to a method for treating tobacco stems with micrococcus acidophilus and improving the aroma. Background technique [0002] Tobacco is a plant of the genus Nicotiana in the family Solanaceae. The tobacco stem is the thick and hard vein of the tobacco leaf, accounting for about 25-30% of the leaf weight. Tobacco stems are by-products of the tobacco industry and are mostly disposed of at present. my country is a big tobacco country, and hundreds of thousands of tons of tobacco stem resources are discarded every year, which not only causes environmental pollution, but also wastes natural resources. Studies have shown that the types of components contained in tobacco stems are basically the same as those of tobacco leaves. After adding expanded shredded stems to the formula, due to its high filling value, the amount of shredded tobacco in cigarettes can be reduced, which not ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B5/16
CPCA24B5/16
Inventor 潘高峰
Owner 江苏浩丰生物科技有限公司
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