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Method for preparation of yeast beta-D-glucan by utilization of molecular assembly solubilizing technology

一种葡聚糖、酵母的技术，应用在酵母β-D-葡聚糖的制备,高纯度的酵母β-D-葡聚糖领域，能够解决葡聚糖溶解度低、限制实际应用、限制生物活性等问题，达到制备条件温和、重大经济效益和环保意义、扩大应用范围的效果

Active Publication Date: 2015-09-16
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2013, the total beer production was 50.615 million tons, and about 1 million tons of brewer's yeast were produced, but most of them were only sold as cheap feed, or directly discharged into the sewer as waste
[0006] In the Chinese invention patent application with the application number 201310003610.2, a method for preparing yeast β-D-glucan is disclosed, but the solubility of the glucan prepared by this method is very low and almost insoluble, which greatly limits It limits its biological activity and also limits its practical application in production.

Method used

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  • Method for preparation of yeast beta-D-glucan by utilization of molecular assembly solubilizing technology

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Embodiment 1

[0040] (1) adding deionized water to the cell wall of Saccharomyces cerevisiae, stirring to obtain a suspension, centrifuging and washing until the supernatant is clear, and collecting the precipitate;

[0041] (2) Add deionized water to the precipitate described in step (1), prepare a solution with a concentration of 15% (w / v) in g / mL, and extract it in a constant temperature water bath shaker at 95°C for 4h , centrifuge at 4500rpm for 10min to collect the precipitate, wash the precipitate twice with water, and centrifuge again at 4500rpm for 10min to collect the precipitate;

[0042] (3) Add deionized water to the precipitation described in step (2) to prepare a solution with a concentration of 20% (w / v) in g / mL, add helicase to the solution for enzymolysis, and enzymolysis conditions The method is: temperature 40°C, addition amount 0.01%, time 3h; then treat at 80°C for 10 minutes, carry out enzymatic inactivation treatment, and obtain enzymatic hydrolyzate;

[0043] (4) T...

Embodiment 2

[0051] (1) adding deionized water to the cell wall of Saccharomyces cerevisiae, stirring to obtain a suspension, centrifuging and washing until the supernatant is clear, and collecting the precipitate;

[0052] (2) Add deionized water to the precipitation described in step (1), prepare a solution with a concentration of 25% (w / v) in g / mL, and extract it in a constant temperature water bath shaker at 95°C for 4h , centrifuge at 5000rpm for 5min to collect the precipitate, wash the precipitate twice with water, and centrifuge again at 5000rpm for 5min to collect the precipitate;

[0053] (3) Add deionized water to the precipitation described in step (2) to prepare a solution with a concentration of 15% (w / v) in g / mL, add neutral protease to the solution for enzymolysis, and enzymolysis The conditions are: temperature 45°C, addition amount 0.05%, time 1h; then treat at 80°C for 10 minutes, carry out enzyme inactivation treatment, and obtain enzymatic hydrolysis solution;

[0054...

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Abstract

The invention provides a method for preparation of yeast beta-D-glucan by utilization of a molecular assembly solubilizing technology. The method comprises the following steps: first, an enzymatic hydrolysate of yeast cell walls is subjected to microjet processing under 70-200MPa, then centrifugation is carried out and precipitate is obtained; second, the precipitate obtained from the first step is resuspended by utilization of ionic liquid, scattering processing is carried out and a solution is obtained, wherein, the ionic liquid is 1-ethyl-3-methylimidazole acetate or 1-allyl-3methylimidazole chlorine salt; third, the solution obtained from the second step is subjected to centrifugation, then ethanol is added, centrifugation is carried out and precipitate is collected; fourth, the precipitate obtained from the third step is resuspended by utilization of water, then centrifugation is carried out, and a liquid supernatant is taken. Preferably, the method also comprises the fifth step: the liquid supernatant obtained from the fourth step is subjected to spray drying processing, and yeast beta-D-glucan powder is obtained. The yeast beta-D-glucan is advantaged by high purity and good dissolvability, and the application scope is widened.

Description

technical field [0001] The invention relates to the technical field of food processing, and relates to a method for preparing yeast β-D-glucan, in particular to a method for preparing soluble high-purity yeast β-D-glucan by using molecular assembly solubilization technology . Background technique [0002] β-D-glucan is a kind of polysaccharide widely present in bacteria, fungi, algae and plants, and one of its main sources is Saccharomyces cerevisiae. Yeast β-D-glucan accounts for 30-60% of the dry weight of yeast cell walls, and has many physiological functions such as enhancing immunity, anti-inflammation, antibacterial, anti-infection, anti-virus, anti-cancer, lowering cholesterol, preventing radiation and healing wounds. my country has very rich yeast resources, and the fermentation industry, especially the brewing industry, can produce a large amount of waste beer yeast every year. In 2013, a total of 50.615 million tons of beer was produced, and about 1 million tons ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12N1/06
CPCC12P19/04C08B37/0024
Inventor 王强刘红芝刘丽石爱民胡晖李亚楠林伟静段玉权高洁刘晓永
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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