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Composition for preventing and treating honeybee microsporidiosis and use thereof

A technology for microsporidia and microsporidia is applied in the field of medicinal use for preventing and treating bee diseases, and in the field of traditional Chinese medicine compositions for preventing and treating bee microsporiasis. effect of treatment

Inactive Publication Date: 2015-09-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Our previous research results show that the extract of Toosendan can effectively inhibit the proliferation of Microsporidia in honeybees, but it is highly toxic to bees, so that the extract of Junzizi has a certain effect on inhibiting the proliferation of Microsporidia in honeybees

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Weigh 10 g of Shijunzi and Toosendan after drying at room temperature, add 100 ml of cold water respectively, and completely immerse the two kinds of Chinese herbal medicines. After standing at room temperature for 2 h, first boil with high heat and then boil with simmer for 10-20 min, then filter to get the filtrate. Then add 100 ml of cold water to the filtered residue, repeat the above frying steps, take the filtrate, combine the two filtrates, and store them in a sealed seal at low temperature. Make Junzi water extract and Toosendan water extract in equal volume and mix with sucrose syrup with a mass percentage concentration of 60% to make a drug-containing syrup with a concentration of 7% by volume, so that the bees infected with microsporidia can eat freely .

[0013] Results: On the 7th day and 13th day after infection, the number of Microsporidium spores in the infected bees fed the syrup containing the drug was 55% and 68% of those in the infected bees fed the...

Embodiment 2

[0015] Weigh 10 g of Shijunzi and Toosendan after drying at room temperature, respectively add 100 ml of cold water, completely immerse the two kinds of Chinese herbal medicines, let stand at room temperature for 2 hours, first boil over high heat, then gently boil for 10- After 20 min, filter the filtrate. Then add 100 ml of cold water to the filtered residue, repeat the above frying steps, take the filtrate, combine the two filtrates, and store them in a sealed seal at low temperature. Make Junzi water extract and Toosendan water extract in equal volume and mix with sucrose syrup with a mass percentage concentration of 60% to make a drug-containing syrup with a concentration of 13% by volume, and allow bees infected with microsporidia to eat freely .

[0016] Results: On the 7th day and 13th day after infection, the number of Microsporidium spores in the infected bees fed the syrup containing the drug was 39% and 35% of the infected bees fed the syrup without the drug, resp...

Embodiment 3

[0018] Weigh 10 g of Shijunzi and Toosendan after drying at room temperature, respectively add 100 ml of cold water, completely immerse the two kinds of Chinese herbal medicines, let stand at room temperature for 2 hours, first boil over high heat, then gently boil for 10- After 20 min, filter the filtrate. Then add 100 ml of cold water to the filtered residue, repeat the above frying steps, take the filtrate, combine the two filtrates, and store them in a sealed seal at low temperature. Make Junzi water extract and Toosendan water extract in equal volume and mix with sucrose syrup with a mass percentage concentration of 60% to make a drug-containing syrup with a concentration of 23% by volume, and allow bees infected with microsporidia to eat freely .

[0019] Results: On the 7th day and the 13th day after infection, the number of Microsporidium spores in the infected bees fed the syrup containing the drug was 40% and 37.5% of those in the infected bees fed the syrup without...

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PUM

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Abstract

The invention provides a traditional Chinese medicine composition for preventing and treating honeybee microsporidiosis. The traditional Chinese medicine composition comprises fructus quisqualis and szechwan chinaberry fruit aqueous extract products according to a volume ratio of 1: 1. The traditional Chinese medicine composition comprising fructus quisqualis and szechwan chinaberry fruit aqueous extract products according to a volume ratio of 1: 1 and sugar syrup with a concentration of 60-70% by mass are processed to form medicine-containing syrup with a concentration of 7-23% according to a certain volume ratio, and the medicine-containing syrup is freely eaten by honeybees suffered from honeybee microsporidiosis. The traditional Chinese medicine composition satisfies a traditional Chinese medicine compatibility principle, realizes drug effect complementary, realizes prevention and treatment on honeybee microsporidiosis and can be used in drugs for preventing and treating honeybee microsporidiosis. The traditional Chinese medicine composition effectively reduces toxicity of szechwan chinaberry fruit in a single agent, has effects of szechwan chinaberry fruit and fructus quisqualis, improved composition treatment effects and does not damage honeybees. The traditional Chinese medicine composition utilizes easily available medical raw materials with a wide source, can be prepared simply and has a low cost. The traditional Chinese medicine composition does not produce drug residue on honeybee products and the environment and does not pollute human and animals and the environment.

Description

technical field [0001] The invention belongs to the field of bee feeding and management, and relates to a traditional Chinese medicine composition for preventing and treating bee microsporidiosis, and a medicinal application for preventing and treating bee diseases. Background technique [0002] Bee microsporidiosis, also known as "bee microsporidiosis", is a disease of the digestive tract of adult bees caused by the microsporidia of honeybees. Microsporidium honeybee mainly infects the intestinal epithelial cells of adult bees, destroys the integrity of the bee intestinal tissue, reduces the digestive capacity of the bee intestine; absorbs the nutrients in the bee body, reduces the physique of the bee, and shortens the lifespan of the bee. When colony disease is mild, the symptoms are not obvious, so it is often ignored. Once severe, it may even cause the collapse of the entire bee colony. Microsporidia of honeybees are mainly (Western) Microsporidia mellifera ( Nosema a...

Claims

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Application Information

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IPC IPC(8): A61K36/58A61P33/00
CPCY02A50/30
Inventor 郑火青陈秀贤胡福良王帅
Owner ZHEJIANG UNIV
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