A novel polymavirus associated with diarrhea in children

A technology with the same identity and the same length, which can be used in the treatment or prevention of MXPyV infection and the identification of anti-MXPyV compounds, and can solve problems such as inability to replicate

Inactive Publication Date: 2015-10-07
RGT UNIV OF CALIFORNIA
View PDF49 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In tumor cells, MCV integrates into the host genome but fails to replicate due to truncating mutations in the viral T antigen (51)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A novel polymavirus associated with diarrhea in children
  • A novel polymavirus associated with diarrhea in children
  • A novel polymavirus associated with diarrhea in children

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0203] The preparation and screening methods of combinatorial chemical libraries are well known to those skilled in the art. Such combinatorial chemical libraries include, but are not limited to: peptide libraries (see, for example, U.S. Patent No. 5,010,175, Furka, Int. J. Pept. Prot. Res. 37:487-493 (1991) and Houghton et al., Nature 354:84-88 (1991)). Other chemical types that generate chemical diversity libraries can also be used. These chemical types include but are not limited to: peptoids (such as PCT publication WO 91 / 19735), coded peptides (such as PCT publication WO 93 / 20242), random biological oligomers (such as PCT publication WO 92 / 00091), benzobis Nitrogen (such as US Patent 5,288,514), various isomers such as hydantoins, benzodiazepines and dipeptides (Hobbs et al., Proc. Nat. Acad. Sci. USA 90: 6909-6913 (1993)), ene Polypeptides (Hagihara et al., J. Amer. Chem. Soc. 114: 6568 (1992)), non-peptide peptidomimetics with a glucose scaffold (Hirschmann et al., J. ...

Embodiment 1

[0237] Example 1: Identification of a new type of human polyomavirus (MXPyV)

[0238] method

[0239] Stool sample collection, nucleic acid extraction and Illumina deep sequencing

[0240] Anonymous samples were collected from 96 children suffering from acute diarrheal diseases in three different Mexican states between 2008 and 2009. Diarrhea is defined as: diarrhea or liquid bowel movements three or more times a day, and the sample is taken before the child is treated with rehydration and antibiotics (if prescribed by the doctor). Virus particles were purified from a stool sample by the following method: A suspension consisting of 1 mL of phosphate buffered saline, 0.1 g glass beads, 100 μL of chloroform and 0.2 g of feces was produced, and the suspension was shaken by a mechanical oscillator for 5 minutes, and then 1,000 g in a centrifuge Centrifuge for 20 minutes, and then recover the supernatant. Then, 500 μL of the supernatant was passed through a 0.45 μm filter and treated ...

Embodiment 2

[0285] Example 2: The prevalence of MX polyoma virus in clinical samples

[0286] A real-time RT-PCR assay targeting the VP1 gene was designed to study the prevalence of MX polyoma virus in clinical samples (Tables 2 and 5). The inclusion of the reverse transcriptase step greatly improved the sensitivity of MXPyV detection (Table 5), presumably because it enhanced the detection of viral mRNA transcripts in infected host cells. The RT-PCR results were confirmed by bands of expected size on gel electrophoresis, melting curve analysis and sequencing. All positive results were also independently confirmed by two additional traditional RT-PCR tests targeting different regions of VP1 and the LT-Ag gene. To detect MXPyV in stool samples of children with or without diarrhea from two continents, the prevalence in Mexico is 12.5% ​​(12 out of 96 cases) and the prevalence in California is 3.3% (18 out of 546 cases). Cases), while the prevalence in Chile is 4.2% (4 out of 96 cases). The ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

Provided is a new polyomavirus, provisionally named MX polyomavirus, (MXPyV). Further provided are cDNA nucleic acid sequences, recombinant proteins, expression vectors and host cells, recombinant anti-MXPyV antibodies, vaccines, compositions, methods of detecting MXPyV, methods for assaying for anti-MXPyV compounds, and methods for treating or preventing a MXPyV infection.

Description

[0001] Cross references to related applications [0002] This application claims the benefits of US Provisional Application No. 61 / 692,170 filed on August 22, 2012 under USC 119(e), which is incorporated herein by reference in its entirety for all purposes. [0003] Declaration of rights to inventions under federally funded research and development [0004] This application was completed with government support from the National Institutes of Health Award numbers R01 AI042801, R01HL 105770, R56AI08953 and R01HL105704. The government has certain rights in this invention. Technical field [0005] The present invention relates to the following inventions: a novel polyoma virus (tentatively named MX polyoma virus (MXPyV)) and its nucleic acid, protein, vaccine, composition, kit, method for detecting and diagnosing MXPyV infection, and for treatment Or methods for preventing MXPyV infection, and methods for identifying anti-MXPyV compounds. Background technique [0006] Polyoma viruses ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/37C07K14/025C07K16/08C12Q1/68G01N33/569
CPCG01N33/56983C07K2/00C07K14/005C07K16/081C12N7/00C12N2710/22021C12N2710/22022C12Q1/701G01N33/57446G01N33/6854G01N33/6893G01N2800/06G01N2800/065G01N2800/24
Inventor C·邱G·于A·格雷宁戈P·伊萨C·F·阿里亚斯J·德 里斯J·帕森奈特S·米勒
Owner RGT UNIV OF CALIFORNIA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap