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Method for screening and judging photoperiod-sensitive Artemia populations

A photoperiodic and sensitive technology, applied in animal husbandry and other directions, can solve the problems of economic value discount, inability to effectively control Artemia, and difficulty in selling Artemia adults, to achieve the effect of increasing speed and increasing economic income.

Inactive Publication Date: 2015-10-21
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of artificial seeds, and the reproductive mode of Artemia cannot be effectively controlled during the breeding process (Artemia has two reproductive modes, namely the oviparous mode: the mother produces dormant eggs; the egg viviparous mode: the mother produces nauplii), in During the breeding period of aquatic animal seed (such as high-grade fish, shrimp, crab, jellyfish, etc.), it is hoped to obtain more nauplii or adults, but the cultured Artemia often produce dormant eggs; However, what is often harvested is a large number of nauplii and their adult artemia. Due to the inconvenience of processing, transportation and use, it is difficult to sell the adult artemia, so the economic value is greatly reduced.

Method used

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  • Method for screening and judging photoperiod-sensitive Artemia populations
  • Method for screening and judging photoperiod-sensitive Artemia populations
  • Method for screening and judging photoperiod-sensitive Artemia populations

Examples

Experimental program
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Effect test

Embodiment 1

[0025] In this example, Artemia eggs from Gahai Lake in China were selected for screening and determining whether they are photoperiod-sensitive Artemia.

[0026]Utilize conventional method to hatch this Artemia egg at first, get each 100 of nauplii after hatching is finished, isolate culture respectively (this example is cultured in 50mL centrifuge tube) in photoperiod L18:D6 and L6:D18, salinity 70ppt, temperature In the water body at 25°C (the photoperiod and temperature are regulated by the photoincubator), cultured for 25 days according to the conventional feeding method, Artemia matured and began to reproduce. According to the experimental ecological operation standard, each group was set up with three replicates. Group.

[0027] Then, under the above-mentioned two kinds of photoperiod culture conditions, the reproductive types of the first births of all adult Artemia participating in reproduction were counted, and the proportion A of female individuals in ovoviviparous ...

Embodiment 2

[0036] In this example, Artemia eggs from Aqikule Lake in China were selected for screening and determining whether they are photoperiod-sensitive Artemia.

[0037] At first utilize routine method to hatch this population Artemia ovum, get each 100 of nauplii after hatching is finished, isolate culture respectively (this example is cultured in 50mL centrifuge tube) in photoperiod L18:D6 and L6:D18, salinity 70ppt, In a water body with a temperature of 25°C (the photoperiod and temperature are regulated by the photoincubator), they were cultured for 30 days according to the conventional feeding method, and the artemia was sexually mature and began to reproduce. Repeat group.

[0038] Then, under two kinds of long and short photoperiod culture conditions, the reproductive types of the first litter of all adult Artemia participating in reproduction were counted, and the proportion A of the female individuals in ovoviviparous mode accounted for all female individuals participating...

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Abstract

The invention discloses a method for screening and judging photoperiod-sensitive Artemia populations. The method comprises the steps of taking the nauplius hatched from wild Artemia eggs and raising them separately in long and short photo period within ecological scope, and calculating the individual proportion breeded in ovoviviparity mode under two photo periods respectively for single factor variance analysis. If PA<0.05, the Artemia has a strong photoperoid sensitivity. Get a PB by the same way based on the sub-generation Artemia of the nauplius hatched from the wild Artemia eggs, and if PA<0.05 and PB<0.01, the Artemia populations has a super strong photoperoid sensitivity; if PA<0.05 and PB<0.05, the Artemia populations has a strong photoperoid sensitivity. The Artemia populations selected based on the invention can be used as a feeding source; by regutating and controlling the breeding mode, the production dilemma of the Artemia farmers is solved; at least 25% economic income is increased; and the speed of Artemia cross breeding is substantially improved.

Description

technical field [0001] The invention relates to the research field of germplasm screening of aquatic economic animals, in particular to a method for screening and determining photoperiod-sensitive Artemia populations. Background technique [0002] Artemia is an important class of economical aquatic crustaceans. Its dormant eggs are easy to store and transport, and the hatched nauplii and adult Artemia are used as open bait for aquatic seedlings. The operation is simple and convenient, and its excellent nutritional value and comparative It is known as "soft gold" because of its high economic value. In recent years, with the rapid development of the aquaculture industry, the demand for Artemia eggs has increased. Stimulated by the high market price of naturally caught Artemia eggs, many inland and coastal farmers have tried to carry out artificial breeding of Artemia. to obtain income. However, due to the lack of artificial good species, and the reproductive mode of Artemia ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王智超武军元贺婷婷程勇周亚飞卢西鹏王晓慧梅京南贺江涛
Owner TARIM UNIV
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