A dual drug-loaded fluorescent magnetic microsphere composite system and its preparation method
A dual drug-loading, magnetic microsphere technology, applied in the field of biomedical material technology and nanomaterials, can solve the problems of no anticancer drugs, poor water solubility of kaempferol, and reduced application effects, and achieve mild experimental conditions and good inhibitory effect. , the effect of reducing toxic side effects
Active Publication Date: 2017-05-17
南京瑞贝西生物科技有限公司
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Problems solved by technology
Although kaempferol has a good anticancer effect, its clinical development and application as an anticancer drug at home and abroad is very limited. The main factor is that kaempferol has poor water solubility, resulting in low bioavailability in vivo and reducing the Its practical effect
[0009] So far, there has been no report on the simultaneous delivery of two anticancer drugs, kaempferol and paclitaxel, using a nano-drug delivery system.
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[0049] Add 5mg kaempferol to 2g oil-based modified Fe 3 o 4 After forming a mixed solution in the magnetic fluid, slowly drop the mixed solution into 50mL 2% bovine serum albumin solution with a syringe, and stir to form a uniform emulsion. Next, the emulsion was ultrasonicated for 15 minutes at a power of 300W, and after the reaction, the product was washed and dried to prepare magnetic kaempferol microspheres, which were sealed and stored for future use.
[0050] Synthesis of aminated graphene quantum dots by hydrothermal method (see: Wang L, Wang YL, Xu T, et al, Naturecommunications, 2014, 5:5357).
[0051] Add 1 mg of paclitaxel (PTX) and 15 mg of graphene quantum dots to 30 mL of PBS phosphate buffer solution with pH = 7.4, ultrasonically disperse the mixture, mix well, stir continuously in the dark, and shake for 24 hours to obtain graphene loaded with paclitaxel Quantum dot solution (PTX / GQDs-NH 2 ); the solution is centrifuged, and the precipitate is vacuum-dried t...
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Abstract
The invention discloses a preparation method of a double drug-loading fluorescent magnetic microsphere composite system. The preparation method comprises the following steps: (1) synthetizing a magnetic kaempferol microsphere according to a sonochemical method; (2) synthetizing an amination graphene quantum dot by a hydrothermal method and achieving loading of paclitaxel; (3) connecting the graphene quantum dot loaded with paclitaxel to the magnetic kaempferol microsphere through aminocarboxylic reaction to obtain the fluorescent magnetic microsphere composite system loaded with a kaempferol drug and a paclitaxel drug. In the prepared fluorescent magnetic microsphere composite system, the graphene quantum dot loads paclitaxel through Pi-Pi physical action, and the magnetic kaempferol microsphere loads kaempferol through physically trapping to achieve double drug-loading. The prepared fluorescent magnetic microsphere composite system loads two anti-cancer drugs of paclitaxel and kaempferol, and kaempferol can remarkably enhance the sensibility of human cervical cancer Hela cells to a chemotherapy drug, namely paclitaxel, and the double drug-loading fluorescent magnetic microsphere composite system has the advantages that the treatment effect can be improved, the generation of the drug resistance is delayed, and toxic or side effects are reduced greatly.
Description
technical field [0001] The invention relates to the fields of biomedical material technology and nanometer materials, in particular to a double-loaded fluorescent magnetic microsphere composite system and a preparation method thereof. Background technique [0002] With the intensification of the aging of the global population and the continuous growth of the world's total population, as well as the increase in cancer-related behaviors year by year, the number of cancer patients worldwide has gradually increased. According to the latest statistics of the World Health Organization in 2011, by 2030, the number of people dying of cancer worldwide will continue to increase by 74%. Chemotherapy is one of the important methods in the treatment of malignant tumors. However, due to the lack of targeting of the traditional dosage forms of chemotherapy drugs currently used in clinical practice, it results in low curative effect and strong side effects. Therefore, enhancing the anti-tu...
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IPC IPC(8): A61K49/00A61K9/16A61K31/352A61K31/337A61P35/00
Inventor 张小娟郝凌云张志颖田湘萍马俊杰蔡文斌王前曦
Owner 南京瑞贝西生物科技有限公司