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A kind of extraction and purification method of vitamin H

A purification method and vitamin technology, applied in the field of vitamin H extraction and purification, can solve the problems of vitamin H extraction and purification process without relevant literature reports and the like

Active Publication Date: 2017-06-30
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, vitamin H is produced by synthetic and fermentation methods in China, but there is no relevant literature report on the extraction and purification process of vitamin H produced by fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Vitamin H fermentation broth 10m 3 , the titer is 2540u / ml.

[0030] 1) Pretreatment: the stirring speed is controlled at 30r / min; firstly, adjust the pH of the fermentation broth to 7.1 with 10% sodium carbonate solution, and let it stand for 20 minutes; secondly, raise the temperature of the vitamin H fermentation broth to 60°C, and then let it stand for 40 minutes; finally, the fermentation broth Cool down to room temperature, add 1kg of adsorbent H2061, control the stirring speed at 100r / min; stir for 1min, let stand for 80min, filter with stainless steel wire mesh, the filtration accuracy is 300μm, and obtain 8.2m vitamin H filtrate 3 ;Rinse the stainless steel metal mesh with deionized water, mix the rinse solution with the vitamin H filtrate, and the volume of the filtrate is 10m 3 , the titer is 2497u / ml, and the yield of pretreatment is 98.3%.

[0031] 2) Dilution: add vitamin H filtrate to deionized water 2m 3 , the volume of filtrate is 12m 3 .

[0032] ...

Embodiment 2

[0040] Vitamin H fermentation broth 10m 3 , the titer is 2365u / ml.

[0041] 1) Pretreatment: the stirring speed is controlled at 35r / min; firstly, adjust the pH of the fermentation broth to 7.2 with 15% sodium carbonate solution, and let it stand for 25 minutes; secondly, raise the temperature of the vitamin H fermentation broth to 65°C, and then let it stand for 45 minutes; finally, the fermentation broth Cool down to room temperature, add 2kg of adsorbent H2061, control the stirring speed at 105r / min; stir for 2min, let it stand for 85min, filter with stainless steel wire mesh, the filtration accuracy is 350μm, and obtain 8.3m vitamin H filtrate 3 ;Rinse the stainless steel metal mesh with deionized water, mix the rinse solution with the vitamin H filtrate, and the volume of the filtrate is 10m 3 , the titer is 2327u / ml, and the yield of pretreatment is 98.4%.

[0042] 2) Dilution: add vitamin H filtrate to deionized water 2.5m 3 , the volume of filtrate is 12.5m 3 .

...

Embodiment 3

[0051] Vitamin H fermentation broth 10m 3 , the titer is 2410u / ml.

[0052] 1) Pretreatment: the stirring speed is controlled at 40r / min; firstly, adjust the pH of the fermentation broth to 7.3 with 20% sodium carbonate solution, and let it stand for 30 minutes; secondly, raise the temperature of the vitamin H fermentation broth to 70°C, and then let it stand for 50 minutes; finally, the fermentation broth Cool down to room temperature, add 3kg of adsorbent H2061, control the stirring speed at 110r / min; stir for 3min, let it stand for 90min, filter with stainless steel metal mesh, the filtration accuracy is 400μm, and obtain 8.1m vitamin H filtrate 3 ;Rinse the stainless steel metal mesh with deionized water, mix the rinse solution with the vitamin H filtrate, and the volume of the filtrate is 10m 3 , the titer is 2379u / ml, and the yield of pretreatment is 98.7%.

[0053] 2) Dilution: add vitamin H filtrate to deionized water 3m 3 , the volume of filtrate is 13m 3 .

[00...

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Abstract

The invention relates to a method for preparing the vitamin H by means of vitamin H fermentation liquor. The method comprises the step that preprocessing, ceramic membrane filtering, ultrafiltration, nanofiltration, crystallization and drying are performed on the vitamin H fermentation liquor to obtain the vitamin H. Stable and efficient production of crude drug vitamin H can be achieved. Meanwhile, by means of the method, the vitamin H yield can be increased, and the method has the advantages that energy is saved, environmental pollution is reduced, operation is easy and convenient, and the working efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of vitamin extraction and synthesis, and in particular relates to a vitamin H extraction and purification method. Background technique [0002] Vitamin H, a water-soluble B vitamin containing sulfur, was discovered more than 100 years ago as a yeast growth factor and subsequently found in egg yolk and liver. Vitamin H, as an enzyme cofactor in the body, participates in carboxylation, decarboxylation and transcarboxylation reactions, and acts as a carrier for transferring carboxyl groups from donors to acceptors. The biological function of vitamin H has been widely used in animal husbandry, medicine, biomedical testing, fermentation industry and other fields since its discovery as a food and cosmetic additive. The annual demand in the global market is relatively high, exceeding 200 tons. [0003] At present, vitamin H is produced by synthetic and fermentation methods in China, but there is no relevant litera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D495/04
CPCC07D495/04Y02P20/10
Inventor 王义周丽娜王文超
Owner 宁夏泰瑞制药股份有限公司