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Lateral flowing detection test paper strip

A detection test, lateral flow technology, applied in the field of biochemical detection, can solve the problems of inability to judge the validity of the kit, inability to obtain a positive control, complex and changeable external environment, etc.

Active Publication Date: 2015-10-28
万冰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application 200480027333.8 discloses a lateral flow detection device and its use method. The device includes structures such as water-absorbing materials, filter layers, and reaction pads, but it cannot provide a true positive control during detection.
[0006] However, such a positive control is generally difficult to ensure its stability during the shelf life (normally requires room temperature for 2 years), and it is impossible to obtain a positive control at the same time as each test sample.
Therefore, it is impossible to judge the effectiveness of each kit when used
Moreover, the external environment is complex and changeable, and the testing equipment and control are stored separately, so it is difficult to ensure the quality synchronization of testing equipment and control

Method used

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  • Lateral flowing detection test paper strip
  • Lateral flowing detection test paper strip
  • Lateral flowing detection test paper strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1A

[0067] Example 1 Group A Streptococcus (Strep A) rapid detection (sandwich method)

[0068] The test strips used in this example are as image 3 shown. The test strips of this example include

[0069] The first binding plate 21: a movable gold-labeled first antibody is provided, and the first antibody is anti-Strep A antigen;

[0070] Detection line 22 (T line): Contains immobilized anti-Strep A secondary antibody, which can form primary antibody-Strep A antigen-secondary antibody complex in the presence of Strep A antigen in the sample, and shows The strip, the first binding plate 21 and the detection line 22 (T line) constitute the detection area 2;

[0071] Capture line 31 (line F): contains immobilized goat anti-mouse antibody to capture all the antibodies on the first binding plate 21, the capture line 31 (line F) constitutes the blocking area 3;

[0072] In the area 411 of the first positive loading area 41, usually but not necessarily, there is the same antibody as ...

Embodiment 2

[0081] Embodiment 2 Morphine (Morphine, MOR) rapid detection (competitive method)

[0082] Such as figure 2 Shown, the present embodiment test strip comprises:

[0083] First binding plate 21: comprising a flowable gold-labeled anti-MOR antibody;

[0084] Detection line 22 (T line): contains immobilized MOR-BSA, the first binding plate 21 and detection line 22 (T line) constitute the detection area 2;

[0085] Capture line 31 (line F): containing immobilized anti-MOR antibody, capture line 31 (line F) constitutes the barrier region 3;

[0086] The first positive loading area 41: contains flowable gold-labeled MOR-BSA;

[0087] Second positive loading area 42: containing immobilized anti-MOR antibody;

[0088] The first positive loading area 41 and the second positive loading area 42 together constitute the positive control area 4 .

[0089] The detection process is:

[0090] Negative sample, the sample does not contain MOR (target substance), after the sample is loaded ...

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PUM

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Abstract

The present invention discloses a lateral flowing detection test paper strip, which is provided with a sample adding area, a detection area, a positive control and a liquid absorbing area, wherein the sample adding area is used for adding a sample to be detected, the detection area is used for indicating whether the sample contains the target substance, the positive control area is used for determining the presence of the target substance in the sample and / or the presence of the activity of the detection reagent adopted as the detection area, the liquid absorbing area is used for driving the liquid from the added sample to flow from the sample adding area to the detection area and the positive control area, the detection area is positioned at the proximal end of the sample adding area, and the positive control area is positioned at the distal end of the sample adding area. According to the present invention, the test paper strip is provided with the positive control of the target substance and the positive control is provided in the dry form so as to ensure the activity and the stability, and the positive control test is performed along with the target substance test, such that the positive control can be adopted as the internal positive control of the lateral flowing detection.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a lateral flow detection test strip. Background technique [0002] Lateral flow rapid assays (lateral flow assays) are designed to detect a wide variety of antigens or antibodies from a sample. [0003] The lateral flow immunochromatographic assay device can load the sample to be tested onto the test strip, and the test strip is loaded with one or more detection antibodies with specific binding affinity for the target. The sample to be tested then flows laterally through the migration membrane to a test line loaded with one or more capture antibodies that also have specific binding affinity for the target. If a target is present in the sample to be tested, the detection antibody can specifically bind to the target at the binding region and can then flow laterally to the test line together with the assay sample. Capture antibodies at the test line can likewise specifically b...

Claims

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Application Information

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IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 不公告发明人
Owner 万冰