Matched cultivation method of stevia rebaudiana triploid hybrids

A cultivation method and technology of Stevia, applied in the field of Stevia triploid breeding, can solve the problems of disorganized seeds, mixed groups, unable to produce stevia seeds in large quantities, etc., and achieve the effects of avoiding hybridization of seedlings and improving yield.

Inactive Publication Date: 2015-11-04
有限会社林平
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Problems solved by technology

If diploid and tetraploid Stevia seedlings are planted in the same field, not only diploid and tetraploid can cross each other, but diploid and tetraploid groups will also pollinate and hybridize each other, so the The seeds produced will be messy, including triploid hybrids, tetraploid an

Method used

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Embodiment Construction

[0013] Steps for Breeding Stevia Triploid Hybrids:

[0014] (1) Mutagenesis of stevia tetraploids: use colchicine to double the mutagenesis of some diploid materials of stevia rebaudiana into tetraploids;

[0015] (2) Cultivate parental lines of Stevia rebaudiana: Diploid and tetraploid Stevia rebaudiana single plant materials are cut and multiplied, and a large number of Stevia rebaudiana with the same genotype and reproductive characteristics from a single plant are bred respectively. a parent strain;

[0016] (3) Hybrid seed production: the tetraploid stevia seed production parent line and the diploid stevia seed production parent line are planted in the same field alternately in rows, and insects are used to cross pollinate each other, and the harvested seeds are Triploid Stevia seeds.

[0017] The method for mutagenesis of stevia tetraploids is as follows: take out the test-tube plantlets on the ultra-clean workbench to remove the leaves, cut into about 1 cm l...

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Abstract

The invention discloses a matched cultivation method of stevia rebaudiana triploid hybrids. The method comprises steps as follows: diploid materials of stevia rebaudiana are subjected to chromosome doubling, individual stevia rebaudiana plant materials of tetraploids and diploids to be subjected to matching hybridization are subjected to cutting propagation respectively, a large quantity of seed production parent strains of tetraploid and diploid stevia rebaudiana with consistent genetypes and reproductive traits are obtained respectively, the two seed production parent strains are planted in rows in the same field alternately for hybrid seed production, and due to the self-incompatible characteristic of the stevia rebaudiana, the stevia rebaudiana triploid hybrids are obtained after natural hybridization. According to the huge plant triploid organs, the leaf yield of the stevia rebaudiana can be remarkably increased with the adoption of the measure.

Description

technical field [0001] A method for supporting breeding of stevia triploid hybrids, relating to stevia triploid breeding technology, more specifically a method of hybridizing diploid stevia and tetraploid stevia to breed triploid stevia hybrid approach. Background technique [0002] Among the crops, many crops have bred triploid high-yield and high-quality new varieties, which have brought breakthrough improvements in crop yield and quality. The specific method is to take watermelon as an example. Homozygous diploid watermelon seeds and homozygous tetraploid watermelon seeds are bred into seedlings, planted alternately on the same piece of land, and let them carry out directional hybridization. The obtained seeds are namely It is a triploid watermelon seed. At present, stevia is produced using conventional varieties or even farm varieties from various places. The yield is low and unstable, and the quality is uneven. Triploid plants have the characteristics of gigantic rep...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
Inventor 林平何克勤胡能兵
Owner 有限会社林平
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