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Extraction method of ginseng polysaccharide

A ginseng polysaccharide and extraction method technology, applied in metabolic diseases, drug combination and other directions, can solve problems such as reducing hypoglycemic effect, and achieve the effect of ensuring color and purity of finished products

Active Publication Date: 2017-04-12
河北亿维日用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ginseng polysaccharide hypoglycemic experimental models all use the intraperitoneal injection route, and the oral administration route will significantly reduce the hypoglycemic effect

Method used

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  • Extraction method of ginseng polysaccharide
  • Extraction method of ginseng polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Crush the ginseng, use 6 times the weight of 80v% ethanol to reflux and extract twice, reflux for 2 hours each time, filter, and take the filter residue for later use;

[0017] 2) The filter residue was reflux extracted twice with 6 times the weight of water, each time for 2 hours, filtered, and the filtrate was collected for subsequent use;

[0018] 3) Concentrate the filtrate to an extract with a relative density of 1.1 at 60°C, add 90v% ethanol to the extract until the ethanol content is 80v% to precipitate, and centrifuge to obtain the precipitate;

[0019] 4) Wash the precipitate with 2 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 2 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 20,000, collect the first concentrate, and then the second The first concentrate passed through a hollow fiber membrane with a molecular weight cut-off of 100,000, and the second per...

Embodiment 2

[0027] 1) Ginseng is crushed, refluxed and extracted with 12 times the weight of 95v% ethanol for 3 times, refluxed for 3 hours each time, filtered, and the filter residue is taken for later use;

[0028] 2) The filter residue was refluxed and extracted 3 times with 12 times the weight of water, each time for 3 hours, filtered, and the filtrate was collected for subsequent use;

[0029] 3) Concentrate the filtrate to an extract with a relative density of 1.3 at 60°C, add 95v% ethanol to the extract until the ethanol content is 85v% to precipitate, and centrifuge to obtain the precipitate;

[0030] 4) Wash the precipitate with 4 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 4 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 20,000, collect the first concentrate, and then The first concentrate passed through a hollow fiber membrane with a molecular weight cut-off of 100,000, an...

Embodiment 3

[0033] 1) Crush the ginseng, use 10 times the weight of 90v% ethanol to reflux and extract twice, reflux for 2 hours each time, filter, and take the filter residue for later use;

[0034] 2) The filter residue was reflux extracted twice with 10 times the weight of water, extracted for 2 hours each time, filtered, and the filtrate was collected for subsequent use;

[0035] 3) Concentrate the filtrate to an extract with a relative density of 1.2 at 60°C, add 95v% ethanol to the extract until the ethanol content is 85v% to precipitate, and centrifuge to obtain the precipitate;

[0036] 4) Wash the precipitate with 3 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 3 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 20,000, collect the first concentrate, and then the second The first concentrate passed through a hollow fiber membrane with a molecular weight cut-off of 100,000, and th...

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Abstract

The invention discloses a ginseng polysaccharide extraction method. The ginseng polysaccharide extraction method comprises the following steps: 1), ginseng is extracted with alcohol, filter residues are extracted with water, and a filtrate is collected for standby application; 2), the filtrate is precipitated by ethanol, and a precipitate is obtained; 3), the precipitate is washed and dissolved in water, a product is washed again and dissolved in water, a product passes through a hollow fiber membrane with the molecular weight cut-off being 100,000, a first concentrated solution is collected and passes through a hollow fiber membrane with the molecular weight cut-off being 1,000,000, a second permeate liquid and a second concentrated solution are collected respectively, the second concentrated solution is collected and passes through a hollow fiber membrane with the molecular weight cut-off being 1,000,000, a third concentrated solution is collected and passes through a hollow fiber membrane with the molecular weight cut-off being 1,800,000, a fourth permeate is collected, the second permeate liquid and the fourth permeate liquid are combined, centrifuged and filtered, and a filtrate is collected for standby application; (5), activated carbon is added to the filtrate for decoloration, the mixture is filtered, a filtrate is taken and subjected to spray drying, and ginseng polysaccharide is obtained. The ginseng polysaccharide has good blood sugar reducing effect when taken orally.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting ginseng polysaccharides. Background technique [0002] Ginseng is a traditional Chinese medicine in my country, which has curative effects on many diseases. In recent years, it has also achieved good results in the treatment of diabetes. Ginseng polysaccharide accounts for about 20% of the total weight of ginseng, is one of the main active components of ginseng, and has the advantages of safety and non-toxicity. It has been reported in the literature that ginseng polysaccharides can reduce blood sugar levels in hyperglycemic mice by intraperitoneal injection. Japanese scientific and technological workers have isolated 21 kinds of ginseng polysaccharides from Japanese, Korean and Chinese ginseng. These neutral ginseng polysaccharides can significantly reduce the fasting blood sugar content of diabetic mice through intraperitoneal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P3/10
Inventor 文继承
Owner 河北亿维日用品有限公司