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Inoculating culture and detecting method for gathering copper ions through saccharomycete

A technology for domestication and cultivation of copper ions, which is applied in the directions of biochemical equipment and methods, methods using microorganisms, and methods based on microorganisms, and can solve problems such as methods that do not reflect the tolerance of yeast to copper ions for cultivation and domestication.

Active Publication Date: 2015-11-11
洛阳欧科拜克生物技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latter is mainly a simple compound of organic trace elements, the focus is on the product as an organic trace element additive, and it does not reflect the tolerance of yeast to copper ions and how to cultivate and acclimatize it

Method used

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  • Inoculating culture and detecting method for gathering copper ions through saccharomycete
  • Inoculating culture and detecting method for gathering copper ions through saccharomycete
  • Inoculating culture and detecting method for gathering copper ions through saccharomycete

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Embodiment 1

[0036] According to the technical scheme of the present invention, test tube culture of Candida utilis is carried out to obtain Candida utilis seed liquid. The Candida utilis seed is inoculated in 10 Erlenmeyer flask acclimation media with different copper ion concentrations. The culture results are shown in the table 1 shown. It can be seen from Table 1 that when the copper ion concentration is 50ug / mL, the OD600 and the copper ion absorption rate are both high, so the yeast strain cultured at this concentration of copper ion is used as the starting strain for subculture and domestication, and the results of each generation of domestication As shown in table 2. It can be seen from Table 2 that when the fourth generation is passed down, the OD600 value begins to decrease, and the copper ion absorption rate begins to decrease. The fifth and sixth generations successively decline, so it can be determined that the fourth generation is the cultured copper-rich yeast, and the copper...

Embodiment 2

[0042] According to the technical scheme of the present invention, test tube culture of Candida utilis is carried out to obtain Candida utilis seed liquid. The Candida utilis seed is inoculated in 10 Erlenmeyer flask acclimation media with different copper ion concentrations. The culture results are shown in the table 3 shown. It can be seen from Table 3 that when the copper ion concentration is 100ug / mL, the OD600 and the copper ion absorption rate are both high, so the yeast strain cultured at this concentration of copper ion is used as the starting strain for subculture domestication, and the domestication results of each generation As shown in Table 4. It can be seen from Table 4 that when the third generation is passed down, the OD600 value begins to decrease, and the copper ion absorption rate begins to decrease. The fourth and fifth generations successively decline. Therefore, it can be determined that the third generation is the cultured copper-rich yeast, and the coppe...

Embodiment 3

[0048] According to the technical scheme of the present invention, test tube culture of Candida utilis is carried out to obtain Candida utilis seed liquid. The Candida utilis seed is inoculated in 10 Erlenmeyer flask acclimation media with different copper ion concentrations. The culture results are shown in the table 5 shown. It can be seen from Table 5 that when the copper ion concentration is 50ug / mL, the OD600 and copper ion absorption rates are both high. Therefore, the yeast strain cultured at this concentration of copper ion is used as the starting strain for subculture and domestication. The results of each generation of domestication As shown in Table 6. It can be seen from Table 6 that when the fourth generation is passed down, the OD600 value begins to decrease, and the copper ion absorption rate remains unchanged. The fifth and sixth generations continue to decline. Therefore, it can be determined that the fourth generation is the cultured copper-rich yeast. The ac...

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Abstract

The invention relates to an inoculating culture and detecting method for gathering copper ions through saccharomycete. According to the inoculating culture method, at first, inoculating culture media with different copper ion concentrations and a basal culture medium are prepared; the inoculating culture media with the different copper ion concentrations are inoculated with a cultured candida utilis strain solution, and shaking culture is carried out; candida utilis with a high OD600 value and the low content of the copper ions in supernatant liquor is selected from the cultured strain solution to serve as starting strains; a starting strain solution is taken out, the inoculating culture medium with the smallest copper ion concentration is inoculated with the starting strain solution, after shake-flask culture is carried out, the strain solution is absorbed again, the inoculating culture medium with the second smallest concentration is inoculated with the strain solution, and inoculating culture is carried out according to the method; finally, copper ion conversion rates of different generations of the candida utilis are detected, and when the conversion rates of the copper ions are unchanged or reduced, the strains are strains for producing copper gathering yeast. The cultured saccharomycete can grow in the culture medium with the large copper ion concentration, the conversion efficiency of the copper ions is high, and the basis is provided for development of the new function of the yeast.

Description

technical field [0001] The invention relates to a method for cultivating yeast, in particular to a method for acclimating, cultivating and detecting yeast to enrich copper ions. Background technique [0002] With the rapid development of my country's economy and the continuous improvement of people's living standards, the demand for livestock products such as meat, eggs, milk, skins, hair, and feathers is increasing day by day. This has led to the rapid development of my country's animal husbandry industry, the variety of breeding has increased, the scale of breeding has increased, and the cost ratio and output value of feed in the breeding industry have also continued to expand. In 2013, the total output of commercial feed in the country was 193.4 million tons (with an output value of 678.4 billion yuan), which has become a pillar industry of the national economy. Trace metal elements are essential nutrients for animals and participate in various metabolic pathways. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12Q1/02C12R1/72
Inventor 李旺宋洋洋李元晓武晓红丁轲张光勤
Owner 洛阳欧科拜克生物技术股份有限公司
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