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Method for monitoring HOOK effect in immune gold experiment

A HOOK, colloidal gold technology, applied in the field of monitoring, can solve the problems affecting the accuracy of test results, misdiagnosis, false negative results, etc., to achieve the effect of improving detection efficiency and accuracy

Active Publication Date: 2015-11-11
AHEAD BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When the HOOK effect occurs, there will be false negative results, which will affect the accuracy of the test results and cause serious consequences such as misdiagnosis.

Method used

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  • Method for monitoring HOOK effect in immune gold experiment
  • Method for monitoring HOOK effect in immune gold experiment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for monitoring the HOOK effect of colloidal gold immunochromatography in the determination of urinary chorionic gonadotropin (HCG)

[0018] (1) Determination of t and t50

[0019] Take the HCG standard sample with a concentration of 5000mIU / mL, and use the immune result interpretation recorder to continuously monitor the relationship between the T line gray level and time of the immunocolloidal gold chromatography reaction of HCG, see figure 1 As shown, the constant starting value of T-line gray scale determines the complete reaction time t, from figure 1 It can be judged as 10 minutes, and the T line is the time of 50% of the concentration at the time of complete reaction, that is, t50, from figure 1 It can be judged as 2 minutes.

[0020] (2) Monitoring method of HOOK effect

[0021] From this setting, t is 10 minutes, t 50 for 2 minutes. Measure the T line gray value at 2 minutes and 10 minutes respectively, the experimental data are as follows figure...

Embodiment 2

[0025] Method for monitoring the HOOK effect of colloidal gold immunochromatography in measuring ferritin in blood

[0026] (1) t and t 50 determination of

[0027] Take a ferritin standard sample with a concentration of 100ng / ml, and use the immune result interpretation recorder to continuously monitor the relationship between the T line gray level and time of the immunocolloidal gold chromatography reaction of ferritin, see image 3 As shown, the constant initial value of the reaction product determines the complete reaction time t, from image 3 It can be judged as 10 minutes, and the time when the concentration of the reaction product is 50% of the concentration in the complete reaction is t 50 ,From image 3 It can be judged as 3 minutes.

[0028] (2) Monitoring method of HOOK effect

[0029] From this setting, t is 10 minutes, t 50 for 3 minutes. Measure the T line gray value at 10 minutes and 3 minutes respectively, the experimental data are as follows Figure 4...

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Abstract

The invention discloses a method for monitoring HOOK effect in an immune gold experiment. The method comprises the steps of determining T line gray values A50 and A at t50 and t by using a vaccination effect identification recorder; when A / A50 is smaller than 1.05, determining that the HOOK effect exists, and indicating that a sample needs to be detected after dilution and the result multiplies by the dilution ratio; and when A / A50 is greater than 1.05, determining that the HOOK effect does not exist. By adopting the method disclosed by the invention, whether all the immune gold experiments have the HOOK effect or not is rapidly judged, and the detection result is corrected in time, the sample with the HOOK effect is diluted to be redetermined, the result multiplies by the dilution ratio to obtain a final detection result; if the HOOK effect does not exist, the detection result can be directly reported and the sample dilution redetermination is not needed, so that the immune gold detection efficiency and accuracy rate can be greatly improved.

Description

technical field [0001] The invention belongs to a monitoring method, in particular to a method for monitoring the HOOK effect in an immune colloidal gold test. Background technique [0002] Immunocolloidal gold technology is a new type of immunolabeling technology that uses colloidal gold as a tracer marker to apply to antigens and antibodies. Colloidal gold-labeled immunoreactive substances—antigens (or antibodies) and corresponding antibodies (or Antigen) reacts, and carries out chromatography on the nitrocellulose membrane, and then combines with another antigen (or antibody) coated on the nitrocellulose membrane, is immobilized on the nitrocellulose membrane and shows the colloidal gold particle Color (T-line) for quantitative analysis of the specified antibody or antigen in that sample. [0003] Sometimes no T-line color or only slight T-line color is observed in samples containing high concentrations of analyte. The disappearance or reduction of T-line color at high ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/68G01N33/76
CPCG01N33/558G01N33/68G01N33/76G01N2333/59
Inventor 叶涛
Owner AHEAD BIOTECH
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