Rapid paeonia rockii reproduction method

A quick and fast technology of peony purple spot, applied in the field of obtaining complete regenerated plants of peony purple spot, can solve the problems of manpower and financial resources, affected by seasons, etc., and achieve the effects of strong growth of seedlings, short period of seedling emergence, and good market application prospects

Inactive Publication Date: 2015-11-25
GANSU AGRI UNIV +3
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the rapid method of grafting and propagation has been used in production for a long time, but this method has the disadvantages of being affected by seasons and consuming a lot of manpower and financial resources.

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment 1

[0024] Embodiment 1 Purple spot peony rapid propagation method

[0025] The rapid propagation method of purple spot peony comprises the steps:

[0026] 1) Take the healthy seeds of purple spot peony (newly picked and old seeds are acceptable), soak in water for 2-3 days to make the seed coat absorb water and soften, peel off the seed coat, take the complete endosperm and put it on a super-clean workbench with 75% alcohol Disinfect for 30 seconds, rinse with sterile water for 3 times; then disinfect with mercuric chloride for 10 minutes, and rinse with sterile water for 3 times.

[0027] 2) Cut the endosperm with a scalpel, take out the embryo, and inoculate it into the No. 1 medium: B5+6-BA2.5mg / L+CH0.5g / L+sucrose 20g / L+agar 5g / L to induce the peony purpura in vitro Embryo germination. Placed in an artificial climate chamber, culture conditions: light intensity 2000-2500lx, light time 15h / d, temperature in the cultivation room (incubator) between 20°C and 25°C for light-dark...

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PUM

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Abstract

The invention discloses a rapid paeonia rockii reproduction method. The method includes the following steps of firstly, inoculating paeonia rockii germ into a first culture medium, and inducing the isolated paeonia rockii germ to sprout; secondly, transplanting a paeonia rockii seedling cultivated in the first step into a second culture medium, and inducing the paeonia rockii seedling to root; thirdly, inoculating the paeonia rockii seedling cultivated in the second step into a third culture medium to root; fourthly, hardening the completely-rooting isolated seedling cultivated in the third step and then cultivating the seedling in soil for 15 days to 20 days, and moving the seedling out of a nursery garden to be used for field production. Due to an efficient and stable paeonia rockii adult seedling system, the reproduced complete plant of paeonia rockii is obtained through the germ cultivating technology under indoor manual control; the method has the advantages that the seed germination rate and rooting rate are high, the adult seedling outplanting cycle is short, the adult seedling is strong in growth and free of plant diseases and pests, and good market application prospects are achieved.

Description

technical field [0001] The invention relates to a method for rapid propagation of peony purpura, in particular to a method for obtaining complete regenerated plants of peony purpura under indoor artificial control conditions by adopting embryo culture technology. Background technique [0002] Paeoniarockii (S.G.HawetL.A.Lauener)T.HongetJ.J.Li] belongs to Paeoniaceae Paeoniae peony group, a unique ornamental and medicinal plant in my country, with large and beautiful flowers, long and straight pedicel, because of the It is named for the obvious large purple spots at the base, and it is also the most threatened species among all wild peonies. It was listed as a national third-level protected plant in 1987. The root bark of peony purpura is used for medicine. Its seed oil contains extremely rich unsaturated fatty acids and has high oil yield. In 2011, it was recognized as a new generation of woody oil crops in my country. It is concentrated in Lanzhou, Linxia, ​​Lintao and other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 唐红张健欣张珊
Owner GANSU AGRI UNIV
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