Calamus amide compound as well as preparation method and application of calamus amide compound
A kind of calamus amide and compound technology, applied in the field of calamus amide compound and its preparation, to achieve the effect of promoting activity
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Embodiment 1
[0037] Preparation of active ingredient calamus amide compounds (extraction and separation process)
[0038]Herbal decoction pieces of Shichangpu were purchased from Anhui Jiren Pharmaceutical Co., Ltd. (batch number: 120519, specification: 1kg / bag, origin: Anhui), about 20kg, decocted 3 times with 8, 6, and 6 times the amount of water, 1.5h each time , combined extracts, recovered under reduced pressure to a small volume (80L), filtered through D101 macroporous resin column chromatography, ethanol-water (v / v) gradient elution, recovered solvent under reduced pressure, obtained 30%, 50%, 70%, 95% 4 ethanol eluates.
[0039] The 30% ethanol eluate was dispersed in water and adjusted to pH=3-4 with formic acid, extracted three times with an equal volume of n-butanol, and the n-butanol extract was recovered under reduced pressure to obtain a dry extract, which was dissolved in water and subjected to ODS column chromatography (methanol-water gradient elution), obtain 6 components...
Embodiment 2
[0057] The influence of calamus amide compounds of the present invention on 5-hydroxytryptamine transporter (SERT)
[0058] Using stably transfected hSERT-HEK293 cell line, 4-(4-(dimethylamino)phenyl)-1-methylpyridinium (APP + ) is a fluorescent substrate, and the effect of calamus amide compounds on SERT activity is detected on a high-content system.
[0059] 1) Experimental instruments and reagents
[0060] laboratory apparatus:
[0061] High-content Operetta system and Columbus data management and analysis system (PerkinElmer), ultra-clean bench, pipette gun (1000μL, 200μL, 20μL, 10μL, 2.5μL, Eppendorf, USA)
[0062] Reagents and materials:
[0063] Human embryonic kidney cell line HEK293 (Cell Bank of Type Culture Collection Committee, Chinese Academy of Sciences), hSERTpcDNA3 plasmid (Addgene, plasmid15483), MEM medium (Gibco), APP+ (Sigma), Hoechst33342 (CellSignalingTechnology), 96-well plate (Costar3605)
[0064] 2) Experimental operation process
[0065] First es...
Embodiment 3
[0084]
[0085] Preparation method: uniformly mix calamus amide compounds ((E)-feruloyl-γ-butyric acid amide), lactose and starch according to the above ratio, pass through a 200-mesh sieve, and evenly moisten the wetted mixture Dry and sieve, add magnesium stearate, then compress the mixture into tablets, each tablet weighs 250 mg, and the active ingredient content is 10 mg.
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