Light-induced dielectrophoresis technology-based single cell control method

Abstract

The invention discloses a light-induced dielectrophoresis technology-based single cell control method which has the following advantages that the cost is low, and a light-induced dielectrophoresis platform adopted in the method is low in cost; the operation is easy, the whole control process is basically finished automatically, i.e., only well-cultivated cells are required to be placed in a container, and the other operation is finished by software; the efficiency is high, i.e., as the whole control process is finished automatically, the classification of a large quantity of cells can be finished within a short time. By using the method disclosed by the invention, a problem that complex and elaborate electrode machining is required to be performed in a traditional dielectrophoresis chip is solved, and virtual electrodes with different shapes can be generated dynamically by an optical projection device, so that a non-uniform electric field is generated, dielectrophoresis force acts on the cells, and the control on the cells can be realized under the real-time observation by a microscope picture system.

Description

technical field [0001] The invention relates to the field of single cell dynamics, in particular to a single cell control method based on light-induced dielectrophoresis technology. Background technique [0002] Single-cell control is an interdisciplinary frontier formed by the osmosis development between analytical chemistry, biology and medicine. [0003] The existing single-cell control technology mainly includes patch clamp combined with atomic force microscopy. Langer of the University of Tübingen in Germany first tried patch clamp and atomic force microscopy (AFM) in 1997. Zhang of New York University used the patch clamp / atomic force microscope system in 2001 to study the unique membrane movement, membrane potential and ion current measurement functions of cells. In 2008, Pamir et al. from the University of Munich in Germany combined atomic force microscopy with planar patch clamp technology to study the regulatory relationship between external mechanical stimuli and...

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Problems solved by technology

[0007] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a single-cell control method based on light-induced dielectrophoresis technology, aiming at solving the problem that the existing single-cell control method is very cumbersome, costly, time-consuming and has no vision. Feedback question

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