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Rapid propagation method of cynanchum thesioides

A sweet potato melon and fast technology, which is applied in the field of rapid propagation of sweet potato melon, can solve the problems of low yield of sweet potato melon, and achieve the effects of fast growth rate, high survival rate, and large-scale development.

Inactive Publication Date: 2015-12-02
安庆市枞阳县阳和苗圃
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of low yield of sweet potato in the prior art, the present invention provides a method for rapid propagation of sweet potato

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of rapid propagation method of sweet potato, comprising the following steps:

[0017] (1) Material acquisition and processing: cut 2 cm stems with terminal buds from the year-old sweet melon plants that are free from diseases and insect pests, remove the leaves, rinse with tap water for 20 seconds, and then use 70% alcohol to disinfect the surface of the stems for 30 seconds , then rinse the sterilized stem section 4 times with sterile water;

[0018] (2) Bud cluster induction: Inoculate the stem segments obtained in step (1) in the improved MS+NAA0.3mg / L+6-BA1.2mg / L+2g / LAC medium for cultivation, add sucrose 20g / L, agar 6.5 g / L, pH5.8, light 3000lx, temperature 25°C;

[0019] (3) Proliferation of clustered buds: after 30 days, the clustered shoots without callus at the base in step (2) were cut off, inoculated on a new medium for proliferation and culture;

[0020] (4) rooting induction: get the clustered shoots subcultured in step (3) and put them into new MS...

Embodiment 2

[0025] A kind of rapid propagation method of sweet potato, comprising the following steps:

[0026] (1) Material acquisition and processing: cut 3 cm stems with terminal buds from the year-old sweet potato plants without pests and diseases, remove the leaves, rinse with tap water for 25 seconds, and then disinfect the surface of the stems with 70% alcohol for 30 seconds , then rinse the sterilized stem section 6 times with sterile water;

[0027] (2) Bud cluster induction: Inoculate the stem segments obtained in step (1) in the improved MS+NAA0.3mg / L+6-BA1.2mg / L+2g / LAC medium for cultivation, add sucrose 20g / L, agar 6.5 g / L, pH5.8, light 3000lx, temperature 25°C;

[0028] (3) Proliferation of clustered buds: after 40 days, the clustered shoots without callus at the base in step (2) were cut off, inoculated on a new medium for proliferation and cultivation;

[0029] (4) rooting induction: get the clustered shoots subcultured in step (3) and put them into new MS medium for 3 t...

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PUM

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Abstract

The present invention relates to the field of nursery and herb cultivation, and in particular to a rapid propagation method of cynanchum thesioides. The method comprises the following steps of: (1) material obtaining and treatment, (2) multiple shoots induction, (3) multiple shoots proliferation, (4) rootage induction, (5) root transplanting, and (6) final-period management. According to the present invention, the method is simple and easy in implementation, high in survival rate, fast in growing speed, so that a large amount of cynanchum decipiens schneid seedlings can be obtained in a short period of time, thus helping the large-scale development of cynanchum decipiens schneid resources.

Description

technical field [0001] The invention relates to the fields of nursery and herbal medicine cultivation, in particular to a rapid propagation method of sweet potato. Background technique [0002] Dixiaogua is a perennial wild herbaceous plant of the family Ascariaceae and the genus Goosedown Vine, about 20 cm high, multi-branched, opposite single leaves, linear leaves, 30-50 mm long, 25 mm wide, small flowers, yellow-white, flowering period 5 From August to October, the fruit period is from August to October. The follicles are spindle-shaped, with short pointed ends and a wide middle part, about 6 cm long and 2 cm wide. There is white latex. When the follicles are light green, wash them and serve as a dish It can be eaten directly, sweet, tender and refreshing, and it is also a delicious food that children have been looking for for thousands of years. In addition, the whole herb and fruit of Di Shaogua can be used as medicine to treat sore throat, dizziness and insomnia, post...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 许宜和
Owner 安庆市枞阳县阳和苗圃
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