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Preparation method of an electrochemiluminescence sensor of a nanocomposite material

A nano-composite material and nano-material technology, which is applied in the field of electrochemiluminescence sensor preparation, can solve the problems of complicated operation process, expensive instruments and equipment, complicated data processing, etc., and achieves good biocompatibility, wide detection range and specific surface area. big effect

Active Publication Date: 2018-02-02
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these traditional analysis methods have strong specificity and high sensitivity, they also have some shortcomings, such as: complex operation process, long time-consuming, complicated subsequent data processing and expensive equipment, etc.

Method used

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  • Preparation method of an electrochemiluminescence sensor of a nanocomposite material

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Experimental program
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Effect test

Embodiment 1

[0028] experimental reagent

[0029] Luminol (>98%) was purchased from Shanghai Biological Biotechnology Co., Ltd. Chlorauric acid (HAuCl 4 ·3H2O) was purchased from Sann Chemical Technology (Shanghai) Co., Ltd. (Annaiji). Absolute ethanol (CH 3 CH 2 OH, AR), hydrazine hydrate (N 2 h 4 ·H 2 O) and glutaraldehyde (25%, GA) were purchased from Shanghai Chemical Reagent Co., Ltd. Bovine serum albumin (BSA, purity>99.8%, molecular weight 68000) was purchased from Xiamen Xinglongda Chemical Reagent Co., Ltd. Ethyl-(3-dimethylpropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich. Carcinoembryonic antigen (CEA) and CEA antibody were purchased from Shanghai Lingchao Biological Co., Ltd. The water used in the experiment was deionized water (18.2 MΩ).

[0030] 2. Preparation of Au@BSA nanospheres

[0031] Add 20 mL of chloroauric acid solution (28.43 mM) to 50 mL of 5.0 mg / mL BSA solution, and stir vigorously at room temperature for 10 min...

Embodiment 2

[0044] A method for preparing an electrochemiluminescence sensor of a nanocomposite material, using the following steps:

[0045] (1) Under stirring at room temperature, the HAuCl 4 Solution and bovine serum albumin solution are mixed evenly, and under the condition of vigorous stirring, quickly add hydrazine hydrate, HAuCl 4 1. The weight ratio between bovine serum albumin and hydrazine hydrate is 20:10:5. After the reaction is complete, age for 12 hours. After the obtained product is centrifuged, it is washed alternately with distilled water and ethanol, and finally the washed product Freeze-drying the prepared Au@BSA nanomaterials;

[0046] (2) Using the cross-linking effect of glutaraldehyde, the luminescent substance luminol is chemically cross-linked to the surface of Au@BSA nanomaterials, and the carboxyl groups on the surface of Au@BSA are further acidified to connect the CEA antibody, and the composite material luminol-Au@ BSA-anti-CEA, the specific steps are as fol...

Embodiment 3

[0055] A method for preparing an electrochemiluminescence sensor of a nanocomposite material, using the following steps:

[0056] (1) Under stirring at room temperature, the HAuCl 4 Solution and bovine serum albumin solution are mixed evenly, and under the condition of vigorous stirring, quickly add hydrazine hydrate, HAuCl 4 1. The weight ratio between bovine serum albumin and hydrazine hydrate is 25:15:7. After the reaction is complete, age for 12 hours. After centrifuging the obtained product, wash it alternately with distilled water and ethanol, and finally wash the product Freeze-drying the prepared Au@BSA nanomaterials;

[0057] (2) Using the cross-linking effect of glutaraldehyde, the luminescent substance luminol is chemically cross-linked to the surface of Au@BSA nanomaterials, and the carboxyl groups on the surface of Au@BSA are further activated to connect the CEA antibody, and the composite material luminol-Au@ BSA-anti-CEA, the specific steps are as follows:

...

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Abstract

The invention relates to a preparation method of an electrochemiluminescence sensor of nano-composite material, which includes preparing Au@BSA nano-material; using glutaraldehyde to cross-link luminol to the surface of Au@BSA nano-material, and activating carboxyl groups on the surface of Au@BSA Connect the CEA antibody to obtain luminol‑Au@BSA‑anti‑CEA; use the electrodeposition method to electrodeposit a gold nanolayer on the surface of the bare glassy carbon electrode of the working electrode, and connect the obtained composite material luminol‑Au@BSA‑anti‑CEA to Three steps to obtain an electrochemiluminescence immunosensor with targeting effect on the antigen CEA on the surface of the electrode. The invention is fast and simple, and the constructed immune sensor has the advantages of good biocompatibility, strong specificity, high sensitivity and wide detection range.

Description

technical field [0001] The invention relates to an electrochemiluminescence sensor, in particular to a preparation method of an electrochemiluminescence sensor of a nanocomposite material. Background technique [0002] Cancer is a major disease that threatens human health. According to the survey of relevant departments, malignant tumors rank second among the causes of death in my country. At present, there is still a lack of effective treatment methods to overcome cancer in the world. If effective early diagnosis of cancer can be achieved, it will be of great significance to later treatment. Early diagnosis is the main way to reduce the mortality rate of malignant tumors. Tumor markers are chemical substances that reflect the existence of tumors. Their content in tumor tissues is much higher than that in normal tissues. Their existence or quantitative changes can indicate the nature of tumors, so as to understand the occurrence and development of tumor tissues. Cell diffe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 贾能勤章阿敏黄楚森
Owner SHANGHAI NORMAL UNIVERSITY
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