Identification and evaluation method of cold tolerance in germination period of momordica charantia

Abstract

The invention discloses an identification and evaluation method of cold tolerance in the germination period of momordica charantia. The germination force, germination rate, vigor index and hypocotyledonary axis length of a material at low temperature are determined, and then the cold tolerance in the germination period of momordica charantia is identified and evaluated through principal component analysis in combination with cluster analysis. Because multiple indexes closely related to the germination period are adopted at the same time, the essential tolerance of momordica charantia can be truly reflected when tolerance evaluation is performed on a large quantity of materials through the method; because the principal component analysis is adopted in combination with cluster analysis for scientific comprehensive evaluation, identification accuracy and reliability are improved, and the method is suitable for identification and evaluation of the cold tolerance of different momordica charantia resources in the germination period and beneficial for accelerating breeding of cold-tolerant momordica charantia varieties.

Description

technical field [0001] The invention belongs to the technical field of identification and evaluation of germplasm resources of bitter gourd, and in particular relates to a method for identification and evaluation of cold tolerance of bitter gourd in germination stage. Background technique [0002] Bitter gourd (Momordicacharantia L.) originated in the tropical region of Southeast Asia and is widely distributed in tropical, subtropical and temperate regions. It likes high temperature and is a cold-sensitive vegetable. The optimum temperature for germination is about 30°C. It germinates slowly below 20°C and germinates below 13°C. difficulty. In early spring cultivation, artificial heating can be used to accelerate germination and raise seedlings. However, in poor areas, seedling cultivation facilities are simple, and the germination temperature is not well controlled, which will easily cause low seed germination rate, reduce seed utilization rate, and increase production cost...

AI Technical Summary

Problems solved by technology

For cold tolerance breeding, the first thing to do is to identify the cold tolerance of the breeding materials. At present, a single index is used for the identification of cold tolerance in the bud stage of bitter gourd, and the index formed by the germination method used cannot reflect that the seeds have the ability to form normal seedlings, so it cannot be reflected objectively. There are certain limitations in the cold tolerance of resources in bud stage

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