In-vitro molecular detection method for manganese SOD2 and primer

Abstract

The invention relates to an in-vitro molecular detection method for manganese SOD (Super Oxide Dismutase) 2 and a primer. The method comprises the following steps of: (1) extracting total RNA (Ribonucleic Acid) from target tissues or cells; (2) using the RNA extracted in the first step for reverse transcription PCR (Polymerase Chain Reaction) to obtain template RNA; and (3) using the primer related in the claim 1 and the template RNA in the second step for real-time fluorescent quantitative PCR. The invention develops a relative quantitative detection kit using the fluorescent quantitative PCR detection as the main detection measure; the expression quantity difference of human SOD2 genes in different tissues and cells can be stably, specifically and accurately detected; and the repeatability is high. The defects of great influence by artificial factors, poor stability, poor repeatability and the like of common-use enzyme activity detection kits in the market are overcome.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for accurate measurement of changes caused by specific proteins called Sodium D-231 Sulfatase (SDS) or other sulfur metabolizing enzymes found naturally within animal body systems like blood vessels walls. By measuring this change with certain techniques, researchers are able to determine whether they have developed any health issues associated with these substances. Overall, this new assay system provides an effective way to measure how well people live without getting sick from common illnesses.

Problems solved by technology

Technologies described in this patents involve measuring small amounts of certain compounds called sulfite oxydisulfonate(SODI), known as redoxyradionuclide, commonly referred to as hydrogen donors during chemotheraphy procedures. However, these techniques may be affected by changes made naturally occurring within individuals' bodies, leading to errors in interpretation and assessment of their effects. Therefore, they cannot accurately identify individual levels of SODO involved in cellular injury processes like apoptosis and necrosis.

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