In-vitro molecule detection method of EC-SOD (extra-cellular super oxide dismutase) and primers

An EC-SOD, molecular detection technology, applied in the field of molecular biology, to overcome the large influence of human factors and the effect of high repeatability

Active Publication Date: 2015-12-16
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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Problems solved by technology

[0007] At present, the market has not yet found a molecular level detection kit related to SOD3, and the present invention provides a strong basis for future molecular detection of SOD

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  • In-vitro molecule detection method of EC-SOD (extra-cellular super oxide dismutase) and primers
  • In-vitro molecule detection method of EC-SOD (extra-cellular super oxide dismutase) and primers
  • In-vitro molecule detection method of EC-SOD (extra-cellular super oxide dismutase) and primers

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Embodiment Construction

[0022] specific implementation

[0023] In order to understand the present invention, the present invention will be further described below in conjunction with embodiment: following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0024] In the design of fluorescent quantitative PCR primers in this method, first, according to the RNA sequence number: NM_003102, the forward primers are all designed across genomic introns, which reduces the contamination of genomic DNA remaining in the sample, resulting in low accuracy of the results And the shortcomings of poor repeatability, improve the specificity and accuracy of the results. Secondly, using NCBIblast to compare with using PRIMER5.0 design software to analyze, optimize primers, control all indications within the optimal range, and greatly reduce the formation of primer dimers. There is no need to add DNase treatment, and the sybergreen method is us...

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Abstract

The invention relates to an in-vitro molecule detection primer of EC-SOD (extra-cellular super oxide dismutase). According to the in-vitro molecule detection primer of EC-SOD, a forward primer sequence is represented in a sequence 1, and a reverse primer sequence is represented in a sequence 2; according to an internal reference matched with the in-vitro molecule detection primer, represented in a claim 1, of EC-SOD, a forward primer sequence is represented in a sequence 3, and a reverse primer sequence is represented in a sequence 4. A relative quantification detection kit giving priority to fluorescent quantitation PCR (polymerase chain reaction) detection is developed, expression difference of SOD3 genes in different tissues and cells of a human body can be detected stably, specifically and accurately, extremely high repeatability is achieved, and the defects of high influence of human factors, poor stability, poor repeatability and the like of a common enzyme activity detection kit in the market are overcome. On the basis of cooperation with a large quantity of clinical experimental data in the fields of medical detection and senescence resistance of cosmetics, the conditions of related diseases reflected by expression quantity of SOD3 enzyme can be judged at the early stage, and positive effects on early treatment and prevention of the diseases is realized.

Description

technical field [0001] The invention belongs to the field of molecular biology and is mainly used in in vitro experiments and clinical in vitro diagnosis, in particular an in vitro molecular detection method and primers for EC-SOD. technical background [0002] SOD is the abbreviation of SuperOxide Dismutase, and the Chinese name is superoxide dismutase. It is an important antioxidant enzyme in organisms and is widely distributed in various organisms, such as animals, plants, and microorganisms. SOD has special physiological activity and is the primary substance for scavenging free radicals in organisms. The level of SOD in the living body means an intuitive indicator of aging and death; it has been confirmed that there are as many as 60 kinds of diseases caused by oxygen free radicals. It can resist and block the damage to cells caused by oxygen free radicals, repair damaged cells in time, and reduce the damage to cells caused by free radicals. Due to the pressure of mode...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6851C12Q1/6883C12Q2600/158C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 孙麟马洁刘宇
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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