Method for inducing efficient accumulation of DHA in Chlorella vulgaris ZF strain

Abstract

The invention provides a method for inducing the efficient accumulation of DHA in Chlorella vulgaris ZF strains, which is characterized in that the following steps are adopted: 1) preparation of algae liquid: at 23°C±2°C, light intensity 1900±100lx and light-dark ratio of 12h / Under the condition of 12 hours, use 1 / 4 times of BG11 medium to cultivate the cells of Chlorella vulgaris ZF algae strain for 28 days to the logarithmic growth phase, and obtain the algae liquid used for plant growth regulator induction, and add 1 double concentration of BG11 nutrient salts; 2) Accumulation of DHA: Take 200mL algae liquid in the logarithmic growth phase and place it in a 300mL triangular conical flask, then add the plant growth regulator 6-BA, and the plant growth regulator 6-BA in the algae liquid The concentration of DHA is 0.01±0.001mg / L, and the induction treatment is carried out at 23°C±2°C for 14±1 days. During the treatment stage, the artificial shaker algae is not less than 3 times a day, and the time interval between adjacent shaker algae is not less than 2 hours, so as to realize the DHA in the Accumulation in algae fluid. The invention is simple and easy to implement, has low cost and can significantly accumulate DHA content in a short period of time.

Description

technical field [0001] The invention provides a method for inducing high-efficiency accumulation of DHA in ZF strains of Chlorella vulgaris, belonging to the field of biotechnology. Background technique [0002] Chlorella (Chlorella) is a common aquatic single-celled algae, which is an important genus in the family Chlorellaceae. It has a wide ecological distribution and is distributed in both seawater and freshwater. Currently, it includes about 10 species. Chlorella cells are rich in protein (50% of dry weight), essential amino acids, polysaccharides, lipids (results of fatty acid accumulation), chlorophyll, carotene, and vitamins, etc., used in health food, aquaculture (feed additives) ), cosmetics, medicine and other fields are widely used, and the domestic and foreign markets are in short supply. Compared with the United States, Japan, Israel and other countries, the level of industrialized large-scale cultivation of Chlorella in my country is relatively lagging behind...

AI Technical Summary

Problems solved by technology

Although the above-mentioned relevant patented technologies related to purification and preparation of DHA are advanced, they do not involve plant growth regulators inducing Chlorella vulgaris to accumulate DHA, and the technical process is relatively complicated and expensive, which undoubtedly increases production costs. and the difficulty of technology promotion