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Multi-sample detection device and method

A detection device and detection method technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of unfavorable mass sample testing, unfavorable sample testing market development, low experimental efficiency, etc., and meet the requirements of fast and accurate sensitivity and meet the sensitivity requirements. , the effect of improving the accuracy

Inactive Publication Date: 2015-12-09
AILEX TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the containers for cultivating bacteria in the experiment are test tubes or 96-well plates. Manual data acquisition and low experimental efficiency are not conducive to large-scale sample testing, and are not conducive to the development of the current sample testing market.

Method used

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  • Multi-sample detection device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] like figure 1 Shown is the schematic cross-sectional structure of the multi-sample detection device. As shown in the figure, the light source and the data acquisition plate are located on both sides of the 96-sample well plate, and the sample well plate is provided with a plurality of sample wells arranged in rows and columns, as shown in the figure. It is a row of a 96-well plate, in which the light source structure includes a light mixing film and a 3-in-1 LED. The light source illuminates multiple sample wells in a row at a time, and the 8-channel silicon photodiode PD on the data acquisition board (located on the PCB board in the figure) is generated by the light source. Detection head detection.

[0085] After the detection of one row is completed, the sample well plate can be moved relative to the light source and the data acquisition plate, so as to detect the samples in each row or all the sample wells of the sample well plate. The structure diagram of the sampl...

Embodiment 2

[0121] The detection data for a row of 8 sample wells are as follows:

[0122]

[0123] Now take a few representative rows of data as an example to illustrate:

[0124] Among them, 55AA58494AAC31090101FE00 is the instruction data area, and 0000 in the last line is the terminator.

[0125] The 8 sample well channels are illuminated by R, G, B three-color light sources in sequence, and the obtained data can be listed in the following table:

[0126] R

00 80

00 80

00 80

0F 80

00 80

00 80

00 80

00 80

G

00 80

00 80

00 80

00 80

00 80

00 80

02 80

00 80

B

03 80

06 80

0A 80

00 80

01 80

18 80

12 80

14 80

hole 1

hole 2

hole 3

hole 4

hole 5

hole 6

hole 7

hole 8

[0127] In addition, if the collected data is 0x0E80=0x000E=14, the actual data = the collected data - 0x8000, the highest bit of the data in the design is used...

Embodiment 3

[0155] Preferably, the method further includes the step of: restoring the color of the sample in each sample well according to the colorimetric value of the sample in each sample well in Example 2.

[0156] Image 6 It is the result of the software complex color display when the bacterial solution is just added to the reagent plate, Figure 7 It is the display result of the color restoration of the detected data by the software after 20 hours, Figure 8 Test plates for bacterial growth taken after 20 hours. Among them, the first column of data in the figure is turbidity, and the other columns are color information.

[0157] The data in each sample hole represents the numerical component of R|G|B, the red component is more red than other colors, the red and green components are both 255, and the blue component 0 is yellow.

[0158] Figure 7 The test data show that its Figure 8 The colors of the samples in the photographed bacterial growth test plate are the same, so the ...

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Abstract

The present invention relates to the field of sample detection, and discloses a multi-sample detection device and method. The detection device includes a sample hole plate, a microcontroller, a strip light source and a data acquisition board; the sample hole plate is provided with a plurality of sample holes which are arranged in rows and used for receiving samples to be tested; the strip light source and the data acquisition board are respectively located both sides of the sample hole plate, the strip light source illuminates in single time a row of n sample holes in total, the data acquisition board corresponding is provided with at least n detection channels; the microcontroller is used for sending light source control signals to the strip light source so as to control the strip light source switching state to start different types of light to illuminate the samples in the sample holes; the microcontroller is used for sending acquisition signals to the data acquisition board and receiving light transmitting quantity data collected by the data acquisition board; the sample hole plate can moves relative to the strip light source and the data acquisition board, so that samples in each row or all of the sample holes of the sample hole plate can be detected.

Description

technical field [0001] The invention relates to the field of sample detection, in particular to a multi-sample detection device and a method thereof. Background technique [0002] In the field of microorganisms and in vitro diagnostics, bacterial identification and drug susceptibility research have received attention. The application mechanism is that different bacteria will cause changes in the color and turbidity of the culture medium during the growth of a specific culture medium. Most of the containers used for culturing bacteria in experiments are test tubes or similar to 96-well plates. Manual data acquisition and low experimental efficiency are not conducive to the detection of large quantities of samples, nor is it conducive to the development of the current sample detection market. [0003] Therefore, there is an urgent need in the art for a new device and method for simultaneously detecting multiple samples, which can improve the application efficiency of this aspe...

Claims

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Application Information

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IPC IPC(8): G01N21/25
Inventor 李子樵张圣贵
Owner AILEX TECH GRP CO LTD
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