Determination method of natural vitamin E content in sunflower oil

A technology of a natural vitamin and a determination method, which is applied in the field of determination of the content of natural vitamin E in sunflower oil, can solve the problems of complicated steps, long time and the like, and achieves simple steps, good accuracy and precision, and egg shrinkage detection test piece. Effect

Inactive Publication Date: 2015-12-09
COFCO CHENGDU GRAIN & OIL IND
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These standards are extracted by saponification method before liquid...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of assay method that detects natural vitamin E content in sunflower oil, comprises the following steps:

[0026] (1) Weighing: In parts by weight, take 1 sunflower oil sample and weigh it precisely;

[0027] (2) Ultrasonic extraction: add 2~3 parts of diethyl ether extractant, ultrasonically extract for 20~25min, pour out the supernatant and collect;

[0028] (3) Repeat step (2) 2 times;

[0029] (4) Concentration: Collect the supernatant and concentrate to dryness to obtain the sample extract;

[0030] (5) Determination: Determination by high performance liquid chromatography, using C18 chromatographic column, the column temperature is controlled at 33°C, the volume ratio of mobile phase to water is 95:2, isocratic elution is 18min, the flow rate is controlled at 1.2mL / min, diode array The detection wavelength of the detector is 295nm, and the injection volume is 20μL;

[0031] Preparation of reference substance solution: take vitamin E reference substance an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a field of the natural product content determination, and concretely relates to a determination method of natural vitamin E content in sunflower oil. An extraction condition of the natural vitamin E is optimized by using an ultrasonic method, the content of the natural vitamin E in the sunflower oil sample is determined by using a HPLC method, thereby providing reference for plant oil quality standards. The invention has the advantages of simple step, economic utility and easy popularization.

Description

technical field [0001] The invention relates to the field of content determination of natural products, in particular to a method for determining the content of natural vitamin E in sunflower oil. Background technique [0002] Natural vitamin E (VitaminE) is a fat-soluble vitamin, also known as tocopherol, is one of the most important antioxidants. Soluble in organic solvents such as fat and ethanol, insoluble in water, stable to heat and acid, unstable to alkali, sensitive to oxygen, not sensitive to heat, but the activity of vitamin E is significantly reduced when fried. Tocopherol can promote the secretion of sex hormones, increase the vitality and quantity of sperm in men; increase the concentration of estrogen in women, improve fertility, prevent miscarriage, and can also be used to prevent and treat male infertility, burns, frostbite, capillary bleeding, menopausal syndrome, Beauty and other aspects have a very good curative effect. In recent years, it has also been ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06
Inventor 陈海伟西芳李俊金卫华姜凯旋
Owner COFCO CHENGDU GRAIN & OIL IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap