Squamous cell carcinoma antigen measurement system

Abstract

The invention relates to a squamous cell carcinoma antigen measurement system, which includes magnetic beads coated by an anti-fluorescein isothiocyanate antibody, a squamous cell carcinoma antigen antibody marked by the fluorescein isothiocyanate, a squamous cell carcinoma antigen antibody marked by N-(aminobutyl)-N-(isoluminol), a chemical luminescent substrate and a fluorescent detection device. The squamous cell carcinoma antigen antibody marked by the fluorescein isothiocyanate, the squamous cell carcinoma antigen antibody marked by the N-(aminobutyl)-N-(isoluminol) and a to-be-test substance form a bis-antibody sandwich composite. The magnetic beads coated by the anti-fluorescein isothiocyanate antibody is used for being combined with the bis-antibody sandwich composite to form an antigen antibody composite precipitate which is subjected to a fluorescent reaction with the chemical luminescent substrate. The fluorescent detection device is used for detecting fluorescent intensity of the fluorescent reaction. The squamous cell carcinoma antigen measurement system does not contain any radioactive substances, is high in sensitivity, is good in specificity and is high in accuracy.

Description

technical field [0001] The invention relates to a squamous cell carcinoma antigen assay system. Background technique [0002] Squamous cell carcinoma antigen (SCCa), a glycoprotein with a molecular weight range of 45-55 kDa isoforms, was first proposed by Kato and Torigoe in 1997 as a tumor-associated antigen. SCCa is normally expressed in skin and other squamous epithelial cells and has been shown to be helpful in the diagnosis of lung cancer, especially squamous cell carcinoma. [0003] High concentrations of SCCa have been reported to be detectable in human head and neck, liver and other epithelial cancer tissues. It has also been reported in the literature that SCCa is overexpressed in the serum, oral cavity, lung, skin, head, neck, esophagus, anal canal and vulva of various cervical cancer patients. For example, SCCA levels were increased by 72% in the serum of patients with cervical cancer and 94% in the serum of patients with squamous cell carcinoma of the lung. De...

AI Technical Summary

Problems solved by technology

However, the ELISA method has its limitations, such as poor specificity, low sensitivity, low accuracy, etc.

Although radioimmunoassay is considered to be an accurate and reliable method, the radiation effects of radioactive isotopes, the need for specialized laboratories for waste disposal, the disadvantages of high cost and short half-life have made this method gradually eliminated.

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