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Detection device for fungal parasitic soybean cyst nematode

The technology of soybean cyst nematode and detection device is applied in the field of agricultural biological experiment and can solve the problem of difficulty in accurately measuring the parasitic situation of the parasitic fungus soybean cyst nematode and the like

Inactive Publication Date: 2017-06-13
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that it is difficult to accurately measure the parasitic conditions of soybean cyst nematodes caused by parasitic fungi in existing laboratories, and to provide a detection device for fungal parasitic soybean cyst nematodes

Method used

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  • Detection device for fungal parasitic soybean cyst nematode
  • Detection device for fungal parasitic soybean cyst nematode
  • Detection device for fungal parasitic soybean cyst nematode

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Experimental program
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specific Embodiment approach 1

[0011] Specific embodiment one: the present embodiment fungus parasitic soybean cyst nematode detection device is made up of detection tube 1 and cell culture plate 2, and wherein detection tube 1 is to add a cap 1-2 on the upper end of tube body 1-1, tube A round hole 1-3 is opened on the top surface of the cover 1-2, and a through hole 1-4 is opened at the lower end of the tube body 1-1. The detection tube 1 is placed in the culture tank 2-1 in the cell culture plate 2, The upper surface of the detection tube 1 tube cover is covered with two layers of lens cleaning paper 3, and the culture tank 2-1 is filled with ZnCl 2 solution or sterile water, ZnCl 2 Add the solution or sterilized water to the height of lens cleaning paper 3.

[0012] In this embodiment, the through hole at the lower end of the tube body plays a role of ventilation, which can make it easier for the liquid in the culture plate to contact the lens cleaning paper of the detection tube.

[0013] This embodi...

specific Embodiment approach 2

[0014] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the height of the pipe body 1 - 1 is 12-15 mm.

specific Embodiment approach 3

[0015] Embodiment 3: This embodiment is different from Embodiment 1 or Embodiment 2 in that the inner diameter of the pipe body 1 - 1 is 6-20 mm.

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Abstract

The invention discloses a fungus parasitic soybean heterodera glycines ichinohe detecting device, belongs to the field of agricultural bio-experiments, and aims at solving the problem that an existing laboratory can hardly accurately detect the parasitism condition of parasitical fungi on soybean heterodera glycines ichinohe in different insect states. The fungus parasitic soybean heterodera glycines ichinohe detecting device is composed of a detecting tube and a cell culture plate. According to the detecting tube, a tube cover is arranged on an upper end cover of a tube body, a round hole is formed in the top face of the tube cover, a through hole is formed in the lower end of the tube body, the detecting tube is arranged in a culture trough in the cell culture plate, the upper surface of the tube cover of the detecting tube is covered with two layers of lens wiping paper, and the culture trough is filled with a ZnCl2 solution or sterilization water. The detecting device can accurately detect the parasitism condition of parasitical fungi on the soybean heterodera glycines ichinohe in different insect states, the function of parasitical fungi on the soybean heterodera glycines ichinohe in different insect states is visually detected, and a more effective method is provided for screening of biocontrol resources of the soybean heterodera glycines ichinohe.

Description

technical field [0001] The invention belongs to the field of agricultural biological experiments, and in particular relates to experimental equipment for measuring and researching the parasitic conditions of soybean cyst nematodes in different insect states by fungi, screening and measuring soybean cyst nematode bio-control resources. Background technique [0002] Soybean cyst nematode (SCN) is one of the main limiting factors in soybean production in the world. Because of its wide distribution, serious damage, large host range, multiple transmission routes, strong cyst resistance and long survival time, the pathogenic soybean cyst nematode has become a soil-borne disease that is extremely difficult to control. Because biological control is less harmful to the environment and human beings, research on the biological control of soybean cyst nematode has become the focus. [0003] At present, according to the mechanism of action of fungi on soybean cyst nematodes, biocontrol ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34
CPCC12M41/00
Inventor 宋洁许艳丽姚钦
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S