A kind of seed coat specific expression promoter and its application

Abstract

The invention discloses a seed coat-specific expression promoter and its application, belonging to the technical field of plant genetic engineering. The promoter has the nucleotide sequence shown in SEQ ID NO.1, or has the same sequence as shown in SEQ ID NO.1 Sequence-complementary nucleotide sequences. The seed coat-specific expression promoter is a new promoter gene, which can promote the specific expression of the target gene on the seed coat of the plant, and realize the spatiotemporal specific expression of the foreign gene in the plant; It can drive the expression of the downstream target gene in the seed coat of the plant, thereby avoiding the adverse effects caused by the continuous expression of the target gene in other tissues of the plant; the promoter can also be directly applied to the screening of transgenic plants, and can be realized at the seed stage of the plant Screening has the advantages of strong stages and high screening sensitivity.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a seed coat-specific expression promoter and its application. Background technique [0002] Promoter is an important cis-acting element in the regulation of gene expression. According to the mode of action and function in plant genetic engineering, it can be divided into three types: constitutive promoter, tissue-specific promoter and inducible promoter. At present, the commonly used promoters in plant genetic engineering are constitutive expression promoters, such as the cauliflower mosaic virus (CaMV) 35S promoter used in most dicotyledonous transgenic plants, and the Ubiquitin promoter from maize is mainly used in monocotyledonous transgenic plants and the Actinl promoter from rice. Studies have found that there are some defects in the expression of exogenous genes under the control of constitutive promoters. For example, the continuous and efficient express...

AI Technical Summary

Problems solved by technology

Studies have found that there are some defects in the expression of exogenous genes under the control of constitutive promoters. For example, the continuous and efficient expression of exogenous genes in plants will inevitably consume a large amount of basic substances in plants, resulting in energy load and waste of materials. The additional consumption of substances will inevitably affect and inhibit other metabolic activities in plants, and at the same time cause changes in plant morphology, affecting plant growth and development, etc.

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