Application of shrimp meat offal Zn-chelated peptide

A technology of leftovers and chelated peptides, applied in application, food preparation, food science, etc., can solve the problem of high cost of protein and astaxanthin

Inactive Publication Date: 2015-12-30
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, my country is mainly used to produce chitin, and the recycling of protein and astaxanthin is industrialized due to...

Method used

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  • Application of shrimp meat offal Zn-chelated peptide
  • Application of shrimp meat offal Zn-chelated peptide
  • Application of shrimp meat offal Zn-chelated peptide

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Embodiment

[0023] 1) Preparation of protein hydrolyzate from shrimp processing waste: Grind the leftovers (shrimp head and shell) of frozen shrimp processing with a tissue grinder, add phosphate buffer (0.2mol / L, pH7.0) according to the ratio of solid to liquid 1g: 5mL, and put them under ultrasonic power at 47°C. Sonicate at 500W for 90min, then add neutral protease (enzyme activity ≥ 5.0×10 4 U / g), after incubating at 47°C for 2h, the temperature was raised to 95°C and maintained for 10min; the temperature of the solution was lowered to 37°C, the pH was adjusted to 7.8, and trypsin (enzyme activity ≥ 2.5×10 4 U / g) after 3 hours of heat preservation, the temperature rose to 95 ° C and kept for 10 minutes, at 10000 g Centrifuge for 15 minutes to obtain the supernatant, that is, the proteolyzate of shrimp processing leftovers.

[0024] 2) Preparation of zinc chelating peptide from shrimp processing waste: The prepared enzymatic hydrolyzate is subjected to ultrafiltration treatment wit...

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Abstract

The invention relates to application of a shrimp meat offal Zn-chelated peptide for preparing Zn supplementing food and health food. Particularly, the amino acid sequence of the shrimp meat offal Zn-chelated peptide is Trp-Gly-Phe-Thr-Cys-Trp-Pro-Met, and the detected ESI/MS molecular weight is 1027.20 Da. Due to the unique chelation and transport mechanism of the Zn-chelated collagen peptide, the Zn-chelated collagen peptide is high in absorption and utilization degree, can supplement polypeptide/amino acid and Zn at the same time, and is an ideal Zn supplementing material.

Description

technical field [0001] The invention belongs to the technical field of deep processing of aquatic products, and relates to the application of an ion chelating peptide, in particular to the application of the zinc chelating peptide of shrimp leftovers. Background technique [0002] my country is very rich in shrimp resources. In recent years, with the rapid development of the shrimp processing industry, a large number of processing wastes have been produced, which are rich in effective substances such as protein, chitin and astaxanthin. After investigation, it was found that when the production enterprises that use shrimp as the main raw material process shrimp processing waste, some of them are sold as waste at very low prices, or transported to farms for drying and crushing as feed. The leftovers were eventually transported directly to the garbage dump due to moldy and rotted piles. Not only cause serious waste of resources, but also cause environmental pollution. Foreign...

Claims

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Application Information

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IPC IPC(8): A23L1/305
CPCA23V2002/00A23V2250/542
Inventor 赵玉勤徐银峰王斌陈荫孙坤来
Owner ZHEJIANG OCEAN UNIV
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