Preparation method of chrysophanol proliposome
A technology of proliposome and chrysophanol, which is applied in the direction of liposome delivery, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of uneven dispersion, complicated process, cumbersome operation, etc., and meet the reaction conditions Mild, simple process operation, high drug encapsulation efficiency
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Embodiment 1
[0018] Experimental equipment: rotary evaporator (RE52-99); UV-visible spectrophotometer (TU-1810); circulating water vacuum pump (SHZ-D(III)); low-speed centrifuge (SU-05).
[0019] Experimental reagents: chrysophanol standard substance (National Institute for the Control of Pharmaceutical and Biological Products); chrysophanol test substance (Weiwei Phytochemical Research Institute, Wei'an City, Jiangsu Province); lecithin (Beijing Huaqing Meiheng Natural Products Technology Development Co., Ltd.); cholesterol (Beijing Dingguo Biotechnology Co., Ltd.); PEG-2000 (Beijing Dingguo Biotechnology Co., Ltd.); others are analytical reagents.
[0020] experimental method:
[0021] Dissolve chrysophanol (3mg) in dehydrated ethanol (10mL) containing lecithin (24mg), cholesterol (6mg), PEG-2000 (12mg), vitamin E (10mg) to obtain a mixed solution, and the resulting mixed solution was micro- After filtering and sterilizing the pore filter membrane (pore size is 0.45 μm), inject directly...
Embodiment 2
[0027] Chrysophanol (3mg) was dissolved in absolute ethanol (10mL) containing lecithin (24mg), cholesterol (4.8mg), PEG-2000 (6mg), vitamin E (10mg), to obtain a mixed solution, and the resulting mixed solution was subjected to After microporous filter membrane (0.45μm pore size) is filtered and sterilized, it is directly injected into water (constant volume to 25mL), at room temperature, pH = 7.8, rapidly self-assembles into chrysophanol proliposomes, sealed and kept at 4°C Store at low temperature.
[0028] The detection method for the encapsulation efficiency of the obtained chrysophanol self-assembled proliposomes is the same as in Example 1, and the results are shown in Table 1.
Embodiment 3
[0030] Dissolve chrysophanol (3mg) in absolute ethanol (10mL) containing lecithin (30mg), cholesterol (5mg), PEG-2000 (15mg), vitamin E (10mg) to obtain a mixed solution, and the resulting mixed solution was subjected to micro After filtering and sterilizing through a pore filter membrane (0.45 μm in pore size), inject it directly into water (constant volume to 25 mL), at room temperature, pH=7.8, rapidly self-assemble into chrysophanol proliposomes, seal and store at 4°C save.
[0031] The detection method for the encapsulation efficiency of the obtained chrysophanol self-assembled proliposomes is the same as in Example 1, and the results are shown in Table 1.
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