Method of preparing lipid nano granule

A technology of lipid nanoparticle and diffusion method, which is applied in the direction of liposome delivery and powder delivery, which can solve the problems of particle adhesion and influence on the stability of nanoparticles, and achieve the effect of high drug loading and small particle size

Inactive Publication Date: 2010-04-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although part of the liquid lipid is mixed in the solid lipid material, the prepared nanostructured lipid carrier (NLC) can improve the encapsulation efficiency and drug loading capacity of the drug to a certain extent, and solve the crystallization problem caused by lipid crystallization. The phenomenon of drug extrusion caused by the type change, but the increase of the liquid lipid content will cause the mutual adhesion between the particles, thus affecting the stability of the nanoparticles

Method used

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  • Method of preparing lipid nano granule
  • Method of preparing lipid nano granule

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] see figure 1 , the specific preparation method is as follows:

[0028] 1) Preparation of water-in-oil type submicron emulsion

[0029] The submicron emulsion consists of:

[0030] Double distilled water 14%

[0031] n-Hexane 80%

[0032] Tween 80 5%

[0033] Span 80 1%

[0034] The total weight is 8g.

[0035] Dissolve 400mg of Tween 80 in 1.12g of water to prepare the water phase, and dissolve 80mg of Span 80 in 6.4g of organic solvent to prepare the organic phase. Add the water phase to the organic phase under magnetic stirring and continue to stir for 5 minutes to pre-emulsify. The pre-emulsified emulsion is ultrasonically probed in an ice bath (condition: 400w, work for 2 seconds and stop for 3 seconds, 20 times) to form a water-in-oil submicroemulsion, that is, a nanoreactor;

[0036] The diameter of the formed submicroemulsion droplet was determined to be 75.2m.

[0037] 2) Preparation of lipid nanoparticles by solvent diffusion in submicroemulsion droplet...

Embodiment 2

[0044] 1) Preparation of water-in-oil type submicron emulsion

[0045] The preparation prescription of submicron emulsion is:

[0046] Double distilled water 5%

[0047] n-Hexane 90%

[0048] Tween 80 0%

[0049] Span 80 5%

[0050] The total weight is 8g.

[0051] The specific preparation method is as follows: 400mg Span 80 is dissolved in 7.2g organic solvent to prepare the organic phase, 0.4g pure water is added to the organic phase under the condition of magnetic stirring, and the pre-emulsification is continued for 5 minutes, and the pre-emulsified emulsion is placed on ice Probe ultrasound under bath conditions (condition: 400w, work for 2 seconds and stop for 3 seconds, 20 times) to form a water-in-oil submicroemulsion, that is, a nanoreactor;

[0052] The droplet diameter of the formed submicroemulsion was determined to be 51.6nm.

[0053] 2) Preparation of lipid nanoparticles by solvent diffusion in submicroemulsion droplets (nanoreactor)

[0054]Dissolve 47.5m...

Embodiment 3

[0059] 1) Preparation of water-in-oil type submicron emulsion

[0060] The preparation prescription of submicron emulsion is:

[0061] Double distilled water 12.5%

[0062] n-Hexane 84.75%

[0063] Tween 80 0.25%

[0064] Span 80 2.5%

[0065] The total weight is 8g.

[0066] The specific preparation method is as follows: 20mg Tween 80 was dissolved in 1g water to prepare the water phase, another 200mg Span 80 was dissolved in 6.78g organic solvent to prepare the organic phase, and the water phase was added to the organic phase under the condition of magnetic stirring, and the stirring was continued for 5 minutes Pre-emulsification, the pre-emulsified emulsion is ultrasonically probed in an ice bath (condition: 400w, work for 2 seconds and stop for 3 seconds, 20 times) to form a water-in-oil submicroemulsion, that is, a nanoreactor;

[0067] The droplet diameter of the formed submicroemulsion was determined to be 27.5nm.

[0068] 2) Preparation of lipid nanoparticles by ...

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Abstract

The invention discloses a preparing method of lipid nanometer particle, which comprises the following steps: dissolving O / W typed surface activator in the water to make water phase; dissolving the W / Otyped surface activator into the organic solvent to make organic phase; stirring to add the water phase into organic phase; doing ultrasound for probe under ice bath condition; dissolving the lipid and drug into the alcohol; dripping slowly to stir submicroscopic emulsion; centrifuging to remove organic phase; obtaining the product with small grain size; improving the drug packing rate and drug carrying quantity simultaneously.

Description

technical field [0001] The invention belongs to a method for preparing nanoparticles, and relates to the preparation of lipid nanoparticles and the physical and chemical properties of the prepared lipid nanoparticles. Background technique [0002] Lipid Nanoparticles (Lipid Nanoprticles) is a new type of drug delivery system following emulsions, liposomes, and polymer particles. It is generally prepared from natural or synthetic lipid materials such as complex glycerides and waxes. The particle size is generally in the range of Between 50-1000nm. At present, the commonly used preparation methods of lipid nanoparticles include high-pressure emulsion method, microemulsion method, solvent diffusion method, and emulsification-solvent evaporation method. and other techniques to prepare lipid nanoparticles. Lipid nanoparticles are usually solid at room temperature. They have the advantages of high physical stability and less drug leakage of polymer nanoparticles, and also have t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K9/14A61K47/34A61K47/26
Inventor 袁弘杜永忠胡富强游剑
Owner ZHEJIANG UNIV
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