Ganoderma lucidum spore oil rich in ergosterol and preparation method thereof

A technology of Ganoderma lucidum spore oil and ergosterol, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., and can solve the problems of inability to extract ergosterol components, low extraction rate of triterpenoid components, high cost, etc. problems, to achieve the effect of saving processing costs, saving subsequent processing links, and low cost

Active Publication Date: 2019-03-05
ZHONGKE HEALTH IND GRP JIANGSU PHARM CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process is not only complicated but also expensive
At the same time, the main components of Ganoderma lucidum spore oil obtained in this way are combined organic acids and fatty acids, and the extraction rate of triterpenoids is low, and the ergosterols contained therein cannot be extracted

Method used

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  • Ganoderma lucidum spore oil rich in ergosterol and preparation method thereof
  • Ganoderma lucidum spore oil rich in ergosterol and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Ganoderma lucidum spore oil enriched with ergosterol was prepared as follows,

[0026] (1) Mix 200g of broken Ganoderma lucidum spore powder (source: Ganoderma lucidum spores are produced in Huangshan, and the breaking process is completed by Nanjing Zhongke Pharmaceutical Co., Ltd., requiring a breaking rate > 95%) with 200ml of water, and stir evenly to form a slurry shape;

[0027] (2) Place the slurry obtained in step (1) in an ultrasonic cell disruptor for ultrasonic treatment, the ultrasonic frequency is 25-35MHz, the ultrasonic power is 900-1000W, and the ultrasonic time is 20-30 minutes;

[0028] (3) The ultrasonically treated slurry is filtered by plate and frame, and the filtrate is taken;

[0029] (4) The filtrate is centrifuged at 4000 rpm for 8-12 minutes, and the supernatant is taken to obtain Ganoderma lucidum spore oil rich in ergosterol.

Embodiment 2

[0031] Ganoderma lucidum spore oil enriched with ergosterol was prepared as follows,

[0032] (1) Mix 200g of broken Ganoderma lucidum spore powder (source: Ganoderma lucidum spores are produced in Huangshan, and the breaking process is completed by Nanjing Zhongke Pharmaceutical Co., Ltd., requiring a breaking rate > 95%) with 200ml of water, and stir evenly to form a slurry shape;

[0033] (2) Place the slurry obtained in step (1) in an ultrasonic cell disruptor for ultrasonic treatment, the ultrasonic frequency is 30MHz, the ultrasonic power is 950W, and the ultrasonic time is 25 minutes;

[0034] (3) The ultrasonically treated slurry is filtered by plate and frame, and the filtrate is taken;

[0035] (4) The filtrate is centrifuged at 4000 rpm for 8-12 minutes, and the supernatant is taken to obtain Ganoderma lucidum spore oil rich in ergosterol.

Embodiment 3

[0039] The ganoderma spore oil rich in ergosterol obtained in embodiment 1, embodiment 2 and comparative example 1 is carried out the mensuration of ganoderma triterpenes and ergosterol,

[0040] 1. Determination of ergosterol content

[0041] The content of ergosterol in Ganoderma lucidum spore oil rich in ergosterol was detected according to GB / T 25221-2010 Determination of total ergosterol in grain and oil inspection.

[0042] 2. Determination of content of Ganoderma lucidum triterpenes

[0043] The content of Ganoderma lucidum triterpenoids in Ganoderma lucidum spore oil rich in ergosterol was detected as follows,

[0044] Detection principle

[0045] The triterpenoids in Ganoderma lucidum spore oil rich in ergosterol were extracted with chloroform, and under the action of vanillin-concentrated sulfuric acid, they reacted to form orange substances, which were determined colorimetrically at a wavelength of 500nm within a certain concentration range.

[0046] Testing inst...

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Abstract

The invention relates to the field of traditional Chinese medicines, especially relates to the field of traditional Chinese medicines and health products and concretely relates to ergosterol-rich ganoderma lucidum spore oil and a preparation method thereof. Aiming at ganoderma lucidum spore oil extraction, the novel ergosterol-rich ganoderma lucidum spore oil extraction method is provided and through the extraction method, the ergosterol-rich ganoderma lucidum spore oil with high ergosterol content is obtained. The ergosterol-rich ganoderma lucidum spore oil has ganoderma triterpene content of 40-50wt% and ergosterol content of 0.83wt% or more. The preparation method utilizes water as a dissolving medium, is free of an organic solvent, is suitable for medicine and food industries, is free of a subsequent treatment process and saves a treatment cost. The preparation method is free of carbon dioxide supercritical extraction equipment, has a low cost and high safety and has ganoderma triterpene and ergosterol extraction rates higher than those of the traditional method.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to the fields of traditional Chinese medicines and health care products, and more specifically relates to ganoderma lucidum spore oil rich in ergosterol and a preparation method thereof. Background technique [0002] Ganoderma lucidum is a treasure in the field of traditional Chinese medicine in my country. It is a medicinal fungus of the genus Ganoderma lucidum in the Polyporaceae family. It is included in the "Huangdi Neijing" and is listed as the top grade of traditional Chinese medicine. Li Shizhen, a medical scientist in the Ming Dynasty, recorded in his famous book "Compendium of Materia Medica" that "Ganoderma lucidum is flat in nature, bitter in taste, and non-toxic. ", fully expressed the ancients' deep understanding of the efficacy of Ganoderma lucidum. As a medicinal material, Ganoderma lucidum has been included in the Pharmacopoeia of the People's Republic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/074A61K31/575
Inventor 冯鹏冯敏沈建钱一帆
Owner ZHONGKE HEALTH IND GRP JIANGSU PHARM CO LTD
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