Artemisia rupestris preparation and application thereof in medicaments for treating diabetes
The technology of a diabetes drug and preparation is applied in the preparation and preparation of Artemisia annua in Xinjiang, and the fields of Chinese herbal medicine preparations, which can solve the problems of many side effects and inability to achieve the expected therapeutic effect, and achieve the effect of good effect, good hypoglycemic activity and fast speed.
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Embodiment 1
[0028] Embodiment one: the preparation of Artemisia sativa preparation
[0029] See attached figure 1 , specifically describe the preparation of Xinjiang Yizhi Artemisia preparation as follows:
[0030] (1), first crush the whole Artemisia argyi in Xinjiang into powder, weigh and add 3 times the volume of ether and place it for 8 hours before filtering, and the precipitate is ultrasonically extracted with 80% ethanol at 35°C, each time for 30-40min, and filtered to obtain Artemisia argyi ethanol extract;
[0031] (2) Precipitate the Artemisia argyi extract with 95% ethanol overnight, filter, remove the ethanol by rotary evaporation, extract with ethyl acetate, the extraction temperature is normal temperature, and obtain the ethyl acetate part, and the ethyl acetate part is subjected to polyamide column chromatography Adopt 20%, 40%, 60%, 80% ethanol solutions of different concentrations to elute successively, collect 60% and 80% ethanol eluate and concentrate under reduced p...
Embodiment 2
[0033]Embodiment two: the preparation of Xinjiang Artemisia argyi preparation
[0034] See attached figure 1 , according to the technical scheme described in Example 1, by pulverizing the whole Artemisia chinensis, weighing and adding 3 times the volume of ether, leaving it for 8 hours and then filtering, the precipitate was ultrasonically extracted with 80% ethanol at 35 ° C, each time 30- For 40 minutes, filter to obtain the ethanol extract of Artemisia chinensis, precipitate with 95% ethanol overnight, centrifuge to take the supernatant, extract with ethyl acetate, the extraction temperature is normal temperature, filter to obtain the ethyl acetate part, and the ethyl acetate part is subjected to polyamide column chromatography 20%, 40%, 60%, and 80% ethanol solutions of different concentrations were used for elution in sequence, and the 60% and 80% ethanol eluates were collected, concentrated under reduced pressure into extracts, and the concentrated extracts were combined...
Embodiment 3
[0036] Example 3: Establishment of Experimental Alloxan Diabetic Mouse Model
[0037] Get 120 Kunming male mice with a body weight of 18-22g, feed them adaptively for one week, and randomly select 10 as the normal control group (Nomal control group, NC). Saline configuration), fasting before injection for 12 hours, resulting in an experimental hyperglycemia model, giving 10% glucose water within 48 hours after the model was built, to prevent hypoglycemic death of animals, after 72 hours, blood was taken from the tail of the mice to measure blood sugar (The mice were fasted for 12 hours before blood collection). Observe the drinking water and feed intake of the animals, and whether the typical "three excesses" symptoms of diabetes appear. Finally, the mice whose blood glucose level was ≥11.1mmol / L were selected as experimental diabetic animal models.
[0038] Alloxan can cause damage to islet β cells, thereby affecting the secretion of insulin and increasing blood sugar in an...
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