Preparation method of gypenoside

A technology of Gypenoside and Gynostemma pentaphyllum, which is applied to the preparation of sugar derivatives, chemical instruments and methods, and medical preparations containing active ingredients, to achieve the effects of accelerating the extraction process, reducing yield, and reducing impact

Inactive Publication Date: 2011-01-26
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, this technology is rarely applied to the extraction and separation of traditional Chinese medicine.

Method used

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  • Preparation method of gypenoside
  • Preparation method of gypenoside

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preparation example Construction

[0027] A specific embodiment of a preparation method of gypenoside of the present invention is provided below.

[0028] The device used in the present invention is: at least contain extraction device 1 and expansion bed, extraction device 1 links to each other with infusion pump 2 through pipeline, extraction device 1 links to each other with cylinder 3 of expansion bed through pipeline, infusion pump 2 links to each other through pipeline and The columns 3 of the expanded bed are connected, and the automatic collector 4 is connected with the separation column and the collection container respectively.

Embodiment 1

[0030] (1) Gynostemma pentaphylla medicinal material is pulverized and passed through a 40-mesh sieve, 3 g of Gynostemma pentaphylla fine powder is taken, placed in a 500 ml round-bottomed flask, 150 ml of 40% ethanol extract is added according to a material-to-liquid ratio of 1:50, and an ultrasonic device is turned on for extraction;

[0031] (2) Open the infusion pump 2 while extracting, and the extract is transported from bottom to top in the expanded bed column of the processed HZ-816 macroporous adsorbent resin. The expansion ratio of the column bed is 4: 3; After the pore adsorption resin is adsorbed, the remaining adsorption liquid flows back to the extraction device 1 to continue to participate in the extraction, forming a dynamic extraction of the extraction liquid, so that the effective components are adsorbed on the column bed;

[0032] (3) After extracting for 1 hour, turn off the sonicator, and input 1BV (120ml) deionized water into the cylinder 3 of the expanded ...

Embodiment 2

[0044] In this embodiment, 15g of Gynostemma pentaphyllum medicinal material is used for integrated extraction of total saponins of Gynostemma pentaphyllum by using the device, and the specific operation steps are as follows:

[0045] (1) Gynostemma pentaphylla medicinal material is pulverized and passed through a 40-mesh sieve, 15 g of Gynostemma pentaphylla fine powder is taken, placed in a 500 ml round-bottomed flask, 450 ml of 40% ethanol extract is added according to the ratio of material to liquid at 1:30, and the ultrasonic device is turned on for extraction;

[0046] (2) Open the infusion pump 2 while extracting, and the extract is transported from bottom to top into the expanded bed column of the processed HZ-816 macroporous adsorbent resin. The expansion ratio of the column bed is 3: 2; After the pore adsorption resin is adsorbed, the remaining adsorption liquid flows back to the extraction device 1 to continue to participate in the extraction, forming a dynamic extra...

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Abstract

The invention relates to a preparation method of gypenoside. The method comprises the following steps: placing an extraction solvent and a gynostemma pentaphyllum herb in an ultrasonic extractor for extraction; starting an infusion pump while extracting, transferring extracting solution to a column body of an expanded bed from the bottom up, and allowing the remaining solution after adsorption byresin to return to the ultrasonic extractor to be extracted again; continuously transferring effective constituents of the gynostemma pentaphyllum from the herb to resin in the column during the dynamic extraction process; closing the ultrasonic extractor upon completion of extraction, and transferring flushing liquid to the column body of the expanded bed from the top down by the infusion pump; transferring an eluant into the column body of the expanded bed from the top down by the infusion pump after the flushing liquid becomes clear, and collecting the eluant in an automatic collector; andtransferring another eluant into the column body of the expanded bed from the top of the expanded bed down by the infusion pump upon completion of elution to complete a regeneration process of the column. The preparation method has the advantages of high efficiency, simplified steps, low cost and low energy consumption.

Description

【Technical field】 [0001] The invention relates to the technical field of gypenoside, in particular to a preparation method of gypenoside. 【Background technique】 [0002] Gynostemma pentaphyllum (Thunb) Makino.) is a perennial herbaceous vine of Cucurbitaceae, also known as Aescin, small bitter medicine, etc. Because it has the same effect as Jilin ginseng, it has the reputation of "Southern ginseng" and "Second ginseng", and it is also praised as "green gold" and "Ginseng precious grass" by Chinese and foreign academic circles. Its main active ingredient is gypenoside, which has significant effects of lowering blood sugar, lowering blood fat, balancing blood pressure, anti-cancer, anti-fatigue, anti-hypoxia, anti-heat, anti-aging, promoting cell metabolism, relieving cough and expectorant, improving immunity, whitening, etc. Features. Due to the diversity and importance of gypenoside's physiological activities, optimizing its extraction and separation methods has always be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08A61P11/14A61P3/10A61P9/12A61P39/06A61K36/424A61K125/00A61P39/00A61P11/10A61P35/00A61P3/06A61P29/00A61P37/04
Inventor 胡坪张敏祝付兰罗国安
Owner EAST CHINA UNIV OF SCI & TECH
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