Bacillus thuringiensis YLX-4 and its application

A technology of Bacillus thuringiensis and Bacillus aureus, applied in the field of selenophilic microorganisms and its application, can solve the problems of unidentified microbial species and characteristics

Active Publication Date: 2019-05-03
宁夏硒产业发展有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the invention patent did not isolate and purify related selenium-tolerant microorganisms, nor did it identify the species and characteristics of the microorganisms

Method used

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  • Bacillus thuringiensis YLX-4 and its application
  • Bacillus thuringiensis YLX-4 and its application
  • Bacillus thuringiensis YLX-4 and its application

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Effect test

Embodiment 1

[0036] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0037] Inoculate the microorganism YLX-4 strain of the present invention into the TSB culture solution containing selenium (sodium selenite configuration) 10 mg / L, cultivate it at 200 r / min for 48 hours at normal temperature, and collect the bacteria and supernatant by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and the vast majority of the bacteria are selenomethylselenocysteine ​​(SeMeCys) and selenocystine (SeCys 2 )(Table 2). This shows that YLX-4 bacterial strain can under 10mgSe / L condition, the inorganic selenium (Se 4+ ) into organic selenium (SeMeCys and SeCys 2 ) and accumulate in the bacteria. Further collection of bacteria can be used as organic selenium f...

Embodiment 2

[0042] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0043] The microorganism YLX-4 strain of the present invention is inoculated into the TSB culture solution containing selenium (sodium selenite configuration) 20 mg / L, cultivated at 200 r / min for 72 hours at normal temperature, and the thalline and supernatant are collected by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and most of the bacteria are methyl selenocysteine ​​(SeMeCys) and selenocystine (SeCys 2 ), there is a small amount of Se in the growth plateau of the strain 4+ (table 3). This shows that YLX-4 bacterial strain can under 20mgSe / L condition, the inorganic selenium (Se 4+ ) into organic selenium (SeCys 2 , SeMeCys), and accumulated into the bacteria. F...

Embodiment 3

[0048] The application of the microorganism described in the invention in the synthesis of nanometer selenium.

[0049] The microorganism YLX-4 bacterial strain of the present invention is inoculated in the TSB culture fluid that contains selenium (sodium selenite configuration) 200mg / L, cultivates 24 hours at 200r / min under normal temperature, and the solution appears obvious red, turbid. After culturing for 48 hours, the cells and supernatant were collected by centrifugation, and it was found that the collected precipitate was a red suspension. Then, using liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) and atomic fluorescence spectrometer, it was identified that the composition of the red suspension was almost elemental selenium by subtraction method. The nano-selenium synthesized by the microorganism can be used as a source of selenium supplementation for selenium-deficient people, and can also be used as a selenium fertilizer or an animal feed additive....

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Abstract

The invention discloses bacillus thuringiensis YLX-4 separated from microbial mat sediment in a percolating water system of a selenium mine lot in Enshi, Hubei province of China and an application of the bacillus thuringiensis YLX-4. The strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC No: M2013674. The strain has the selenium-addicted microorganism characteristic, and has potential application value in microorganism organic selenium conversion, microorganism nano-selenium synthesis, selenium-containing ore microorganism activation, selenium-rich microbial fertilizer and selenium polluted environment modification.

Description

technical field [0001] The present invention relates to a selenophilic microorganism and its application, in particular to a strain of Bacillus thuringiensis YLX-4 and its application in microbial transformation and synthesis of organic selenium, microbial synthesis of nano-selenium, microbial activation of selenium ore, selenium-rich microbial fertilizer and selenium polluted environment The application in restoration belongs to the field of biotechnology. Background technique [0002] Extremophiles refer to microbial species suitable for living in extreme environments, including thermophilic bacteria, halophilic bacteria, alkaliphilic bacteria, acidophilic bacteria, barophilic bacteria, psychrophilic bacteria, and radiation-resistant, dry-resistant, and high-concentration metal-resistant bacteria. Ion and extreme anaerobic microorganisms (Rothschild&Mancinelli, 2001; Chen Jun et al., 2006). At present, the research on extremophiles is mainly in areas such as mid-ocean ridg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C12P3/00C05F17/00C02F3/34C12R1/07C02F101/10
CPCY02W30/40
Inventor 袁林喜
Owner 宁夏硒产业发展有限责任公司
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