Selenophilic microorganism wautersiella enshiensis YLX-1 and its application

A technology of YLX-1 and microorganisms, which is applied in the field of selenophilic microorganisms and its applications, and can solve the problems of unidentified microorganism species and characteristics

Active Publication Date: 2018-11-09
SUZHOU SETEK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the invention patent did not isolate and purify related selenium-tolerant microorganisms, nor did it identify the species and characteristics of the microorganisms

Method used

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  • Selenophilic microorganism wautersiella enshiensis YLX-1 and its application
  • Selenophilic microorganism wautersiella enshiensis YLX-1 and its application
  • Selenophilic microorganism wautersiella enshiensis YLX-1 and its application

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Effect test

Embodiment 1

[0052] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0053]Inoculate the microorganism YLX-1 strain of the present invention into the TSB culture solution containing selenium (sodium selenite configuration) 1.5mg / L, cultivate it at 200r / min for 48 hours at normal temperature, and collect the thalline and supernatant by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and most of the bacteria are selenocystine (SeCys 2 )(table 5). This shows that the YLX-1 strain can absorb the inorganic selenium (Se) in the culture medium under the condition of 1.5mgSe / L 4+ ) into organic selenium (SeCys 2 ) and accumulate in the bacteria. Further collection of bacteria can be used as organic selenium for animal feed, etc., and can also be u...

Embodiment 2

[0058] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0059] Inoculate the microorganism YLX-1 strain of the present invention into the TSB culture solution containing selenium (sodium selenite configuration) 10 mg / L, cultivate it at 200 r / min for 72 hours at normal temperature, and collect the bacteria and supernatant by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and most of the bacteria are selenocystine (SeCys 2 ), a small amount of methylselenocysteine ​​(SeMeCys) and Se 4+ (Table 6). This shows that YLX-1 bacterial strain can under the condition of 10mg Se / L, the inorganic selenium (Se 4+ ) into organic selenium (SeCys 2 , SeMeCys), and accumulated into the bacteria. Further collection of bacteria can be used as o...

Embodiment 3

[0065] The application of the microorganism described in the invention in the synthesis of nanometer selenium.

[0066] The microorganism YLX-1 strain of the present invention is inoculated into the TSB culture solution containing selenium (sodium selenite configuration) 100mg / L, and cultivated at 200r / min at normal temperature for 24 hours, and the solution appears red and turbid obviously. After culturing for 48 hours, the cells and supernatant were collected by centrifugation, and it was found that the collected precipitate was a red suspension. Then, using liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) and atomic fluorescence spectrometer, it was identified that the composition of the red suspension was almost elemental selenium by subtraction method. Further use of scanning electron microscopy combined with energy spectrum scattering analysis (SEM-EDS), the results showed that there were a large number of bacteria in the red suspension, and a large num...

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Abstract

The invention discloses a new species of Wautersiella enshiensis YLX-1 isolated from the mat sediment in the percolation water system of Enshi Selenium Mining Area, Hubei, China, and its application. The strain is preserved in the China Typical Culture Collection Center , the deposit number is CCTCC No: M2013671. The strain has the characteristics of selenophilic microorganisms, and has potential application value in microbial organic selenium transformation, microbial nano-selenium synthesis, microbial activation of selenium-containing ore, preparation of selenium-enriched microbial fertilizers, and selenium-contaminated environment restoration.

Description

technical field [0001] The present invention relates to a selenophilic microorganism and its application, in particular to a selenophilic microorganism Wautersiellaenshiensis YLX-1 and its use in microbial transformation and synthesis of organic selenium, microbial synthesis of nano-selenium, microbial activation of selenium ore, preparation of selenium-rich microbial fertilizers and selenium pollution The application in environmental restoration belongs to the field of biotechnology. Background technique [0002] Extremophiles refer to microbial species suitable for living in extreme environments, including thermophilic bacteria, halophilic bacteria, alkaliphilic bacteria, acidophilic bacteria, barophilic bacteria, psychrophilic bacteria, and radiation-resistant, dry-resistant, and high-concentration metal-resistant bacteria. Ion and extreme anaerobic microorganisms (Rothschild&Mancinelli, 2001; Chen Jun et al., 2006). At present, the research on extremophiles is mainly in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C12P3/00C05F17/00C02F3/34C12R1/01C02F101/10
CPCC02F3/34C05F17/00C12N1/20C12P1/04C12P3/00C12N1/205C12R2001/01Y02W30/40
Inventor 袁林喜
Owner SUZHOU SETEK
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